{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:43:08Z","timestamp":1791423788377,"version":"4.3.3"},"reference-count":39,"publisher":"Rockefeller University Press","issue":"8","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,4,17]]},"abstract":"<jats:p>The platelet plays a pivotal role in maintaining vascular integrity. In a manner similar to leukocytes, platelets interact with selectins expressed on activated endothelium. P-selectin glycoprotein ligand 1 (PSGL-1) is the main P-selectin ligand expressed on leukocytes. Searching for platelet ligand(s), we used a P-selectin\u2013immunoglobulin G (IgG) chimera to affinity purify surface-biotinylated proteins from platelet lysates. P-selectin\u2013bound ligands were eluted with ethylenediaminetetraacetic acid. An \u223c210-kD biotinylated protein was isolated from both human neutrophil and platelet preparations. A band of the same size was also immunopurified from human platelets using a monoclonal anti\u2013human PSGL-1 antibody and could be blotted with P-selectin\u2013IgG. Under reducing conditions, both the predicted PSGL-1 \u223c210-kD dimer and the \u223c120-kD monomer were isolated from platelets. Comparative immunoelectron microscopy and Western blotting experiments suggested that platelet PSGL-1 expression is 25\u2013100-fold lower than that of leukocytes. However, patients with chronic idiopathic thrombocytopenic purpura who harbor predominantly young platelets displayed greater expression, indicating that PSGL-1 expression may be decreased during platelet aging. By flow cytometry, thrombin-activated platelets from normal individuals exhibited greater expression than those unstimulated. An inhibitory anti\u2013PSGL-1 antibody significantly reduced platelet rolling in mesenteric venules, as observed by intravital microscopy. Our results indicate that functional PSGL-1 is expressed on platelets, and suggest an additional mechanism by which selectins and their ligands participate in inflammatory and\/or hemostatic responses.<\/jats:p>","DOI":"10.1084\/jem.191.8.1413","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:48:33Z","timestamp":1027702113000},"page":"1413-1422","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":359,"title":["P-Selectin Glycoprotein Ligand 1 (Psgl-1) Is Expressed on Platelets and Can Mediate Platelet\u2013Endothelial Interactions in Vivo"],"prefix":"10.1084","volume":"191","author":[{"given":"Paul S.","family":"Frenette","sequence":"first","affiliation":[{"name":"aThe Center for Blood Research, Department of Pathology,"},{"name":"cDepartment of Medicine, Mount Sinai School of Medicine, New York, New York 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile V.","family":"Denis","sequence":"additional","affiliation":[{"name":"aThe Center for Blood Research, Department of Pathology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linnea","family":"Weiss","sequence":"additional","affiliation":[{"name":"cDepartment of Medicine, Mount Sinai School of Medicine, New York, New York 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Jurk","sequence":"additional","affiliation":[{"name":"eDepartment of Anaesthesiology and Intensive Care Medicine, University of M\u00fcnster, D-48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangeetha","family":"Subbarao","sequence":"additional","affiliation":[{"name":"aThe Center for Blood Research, Department of Pathology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beate","family":"Kehrel","sequence":"additional","affiliation":[{"name":"eDepartment of Anaesthesiology and Intensive Care Medicine, University of M\u00fcnster, D-48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John H.","family":"Hartwig","sequence":"additional","affiliation":[{"name":"bDepartment of Medicine, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietmar","family":"Vestweber","sequence":"additional","affiliation":[{"name":"dInstitute of Cell Biology, Zentrum f\u00fcr die Molekularbiologie der Entz\u00fcndung,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denisa D.","family":"Wagner","sequence":"additional","affiliation":[{"name":"aThe Center for Blood Research, Department of Pathology,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2000,4,18]]},"reference":[{"key":"2023072509180091100_R1","doi-asserted-by":"crossref","first-page":"7450","DOI":"10.1073\/pnas.92.16.7450","article-title":"Platelets roll on stimulated endothelium in vivoan interaction mediated by endothelial P-selectin","volume":"92","author":"Frenette","year":"1995","journal-title":"Proc. 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