{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T09:45:27Z","timestamp":1760607927224},"reference-count":37,"publisher":"Rockefeller University Press","issue":"8","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,10,16]]},"abstract":"<jats:p>Bone marrow (BM)-derived antigen-presenting cells (APCs) are potent stimulators of T cell immune responses. We investigated the requirements for antigen presentation by these cells in priming cytotoxic T lymphocyte (CTL) responses to intracellular bacterial and viral pathogens. [Parent\u2192F1] radiation BM chimeras were constructed using C57BL\/6 donors and (C57BL\/6 \u00d7 BALB\/c)F1 recipients. Infection of chimeric mice with either Listeria monocytogenes or vaccinia virus expressing the nucleoprotein (NP) antigen from lymphocytic choriomeningitis virus (LCMV) primed H2-Db\u2013restricted, but not H2-Kd\u2013restricted CTL responses, demonstrating the requirement for BM-derived APCs for successful priming of CTL responses to these pathogens. Surprisingly, this did not hold true for chimeric mice infected with LCMV itself. LCMV-infected animals developed strong CTL responses specific for both H2-Db\u2013 and H2-Ld\u2013restricted NP epitopes. These findings indicate that in vivo priming of CTL responses to LCMV is remarkably insensitive to deficiencies in antigen presentation by professional BM-derived APCs.<\/jats:p>","DOI":"10.1084\/jem.192.8.1135","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:48:33Z","timestamp":1027702113000},"page":"1135-1142","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":78,"title":["Requirements for Bone Marrow\u2013Derived Antigen-Presenting Cells in Priming Cytotoxic T Cell Responses to Intracellular Pathogens"],"prefix":"10.1084","volume":"192","author":[{"given":"Laurel L.","family":"Lenz","sequence":"first","affiliation":[{"name":"aDepartment of Immunology and the Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric A.","family":"Butz","sequence":"additional","affiliation":[{"name":"aDepartment of Immunology and the Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Bevan","sequence":"additional","affiliation":[{"name":"aDepartment of Immunology and the Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2000,10,16]]},"reference":[{"key":"2023072509290116800_R1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1038\/32588","article-title":"Dendritic cells and the control of immunity","volume":"392","author":"Banchereau","year":"1998","journal-title":"Nature"},{"key":"2023072509290116800_R2","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/S0092-8674(00)80684-4","article-title":"DC-SIGNa guide to some mysteries of dendritic cells","volume":"100","author":"Steinman","year":"2000","journal-title":"Cell"},{"key":"2023072509290116800_R3","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1146\/annurev.immunol.14.1.233","article-title":"CD28\/B7 system of T cell costimulation","volume":"14","author":"Lenschow","year":"1996","journal-title":"Annu. 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