{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T19:18:30Z","timestamp":1793992710219,"version":"4.8.0"},"reference-count":38,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,7,2]]},"abstract":"<jats:p>Proteasomes are the main proteases responsible for cytosolic protein degradation and the production of major histocompatibility complex class I ligands. Incorporation of the interferon \u03b3\u2013inducible subunits low molecular weight protein (LMP)-2, LMP-7, and multicatalytic endopeptidase complex\u2013like (MECL)-1 leads to the formation of immunoproteasomes which have been associated with more efficient class I antigen processing. Although differences in cleavage specificities of constitutive and immunoproteasomes have been observed frequently, cleavage motifs have not been described previously.<\/jats:p>\n               <jats:p>We now report that cells expressing immunoproteasomes display a different peptide repertoire changing the overall cytotoxic T cell\u2013specificity as indicated by the observation that LMP-7\u2212\/\u2212 mice react against cells of LMP-7 wild-type mice. Moreover, using the 436 amino acid protein enolase-1 as an unmodified model substrate in combination with a quantitative approach, we analyzed a large collection of peptides generated by either set of proteasomes. Inspection of the amino acids flanking proteasomal cleavage sites allowed the description of two different cleavage motifs. These motifs finally explain recent findings describing differential processing of epitopes by constitutive and immunoproteasomes and are important to the understanding of peripheral T cell tolerization\/activation as well as for effective vaccine development.<\/jats:p>","DOI":"10.1084\/jem.194.1.1","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:48:33Z","timestamp":1027702113000},"page":"1-12","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":283,"title":["Discrete Cleavage Motifs of Constitutive and Immunoproteasomes Revealed by Quantitative Analysis of Cleavage Products"],"prefix":"10.1084","volume":"194","author":[{"given":"R.E.M.","family":"Toes","sequence":"first","affiliation":[{"name":"cDepartment of Immunohematology and Blood Transfusion, Department of Rheumatology, Leiden University Medical Center, 2333 ZA Leiden, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.K.","family":"Nussbaum","sequence":"additional","affiliation":[{"name":"aInstitute for Cell Biology, Department of Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Degermann","sequence":"additional","affiliation":[{"name":"eBasel Institute for Immunology, CH-4005 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Schirle","sequence":"additional","affiliation":[{"name":"aInstitute for Cell Biology, Department of Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.P.N.","family":"Emmerich","sequence":"additional","affiliation":[{"name":"aInstitute for Cell Biology, Department of Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Kraft","sequence":"additional","affiliation":[{"name":"aInstitute for Cell Biology, Department of Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Laplace","sequence":"additional","affiliation":[{"name":"eBasel Institute for Immunology, CH-4005 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Zwinderman","sequence":"additional","affiliation":[{"name":"cDepartment of Immunohematology and Blood Transfusion, Department of Rheumatology, Leiden University Medical Center, 2333 ZA Leiden, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.P.","family":"Dick","sequence":"additional","affiliation":[{"name":"dSection of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"bBiomathematik, University of T\u00fcbingen, D-72076 T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Sch\u00f6nfisch","sequence":"additional","affiliation":[{"name":"bBiomathematik, University of T\u00fcbingen, D-72076 T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Schmid","sequence":"additional","affiliation":[{"name":"aInstitute for Cell Biology, Department of Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.-J.","family":"Fehling","sequence":"additional","affiliation":[{"name":"fDepartment of Immunology, Medical Faculty\/University Clinics Ulm, D-89070 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Stevanovic","sequence":"additional","affiliation":[{"name":"aInstitute for Cell Biology, Department of Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.G.","family":"Rammensee","sequence":"additional","affiliation":[{"name":"aInstitute for Cell Biology, Department of Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Schild","sequence":"additional","affiliation":[{"name":"aInstitute for Cell Biology, Department of Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2001,6,25]]},"reference":[{"key":"2023072509452642000_R1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1146\/annurev.iy.11.040193.001241","article-title":"Peptides naturally presented by MHC class I molecules","volume":"11","author":"Rammensee","year":"1993","journal-title":"Annu. 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