{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T15:16:13Z","timestamp":1777648573328,"version":"3.51.4"},"reference-count":41,"publisher":"Rockefeller University Press","issue":"10","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,11,19]]},"abstract":"<jats:p>A particular feature of \u03b3\u03b4 T cell biology is that cells expressing T cell receptor (TCR) using specific V\u03b3\/V\u03b4 segments are localized in distinct epithelial sites, e.g., in mouse epidermis nearly all \u03b3\u03b4 T cells express V\u03b33\/V\u03b41. These cells, referred to as dendritic epidermal T cells (DETC) originate from fetal V\u03b33+ thymocytes. The role of \u03b3\u03b4 TCR specificity in DETC's migration\/localization to the skin has remained controversial. To address this issue we have generated transgenic (Tg) mice expressing a TCR \u03b4 chain (V\u03b46.3-D\u03b41-D\u03b42-J\u03b41-C\u03b4), which can pair with V\u03b33 in fetal thymocytes but is not normally expressed by DETC. In wild-type (wt) V\u03b46.3Tg mice DETC were present and virtually all of them express V\u03b46.3. However, DETC were absent in TCR-\u03b4\u2212\/\u2212 V\u03b46.3Tg mice, despite the fact that V\u03b46.3Tg \u03b3\u03b4 T cells were present in normal numbers in other lymphoid and nonlymphoid tissues. In wt V\u03b46.3Tg mice, a high proportion of in-frame V\u03b41 transcripts were found in DETC, suggesting that the expression of an endogenous TCR-\u03b4 (most probably V\u03b41) was required for the development of V\u03b46.3+ epidermal \u03b3\u03b4 T cells. Collectively our data demonstrate that TCR specificity is essential for the development of \u03b3\u03b4 T cells in the epidermis. Moreover, they show that the TCR-\u03b4 locus is not allelically excluded.<\/jats:p>","DOI":"10.1084\/jem.194.10.1473","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:48:33Z","timestamp":1027702113000},"page":"1473-1483","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":38,"title":["T Cell Receptor Specificity Is Critical for the Development of Epidermal \u03b3\u03b4 T Cells"],"prefix":"10.1084","volume":"194","author":[{"given":"Isabel","family":"Ferrero","sequence":"first","affiliation":[{"name":"1Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Wilson","sequence":"additional","affiliation":[{"name":"1Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friedrich","family":"Beermann","sequence":"additional","affiliation":[{"name":"2Swiss Institute for Experimental Cancer Research, CH-1066 Epalinges, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner","family":"Held","sequence":"additional","affiliation":[{"name":"1Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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