{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T01:43:52Z","timestamp":1792892632057,"version":"4.5.0"},"reference-count":21,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,3,4]]},"abstract":"<jats:p>The constraint of fitting a diverse repertoire of antigen specificities in a limited total population of lymphocytes results in the frequency of naive cells specific for any given antigen (defined as the precursor frequency) being below the limit of detection by direct measurement. We have estimated this precursor frequency by titrating a known quantity of antigen-specific cells into naive recipients. Adoptive transfer of naive antigen-specific T cell receptor transgenic cells into syngeneic nontransgenic recipients, followed by stimulation with specific antigen, results in activation and expansion of both donor and endogenous antigen-specific cells in a dose-dependent manner. The precursor frequency is equal to the number of transferred cells when the transgenic and endogenous responses are of equal magnitude. Using this method we have estimated the precursor frequency of naive CD8 T cells specific for the H-2Db\u2013restricted GP33\u201341 epitope of LCMV to be 1 in 2 \u00d7 105. Thus, in an uninfected mouse containing \u223c2-4 \u00d7 107 naive CD8 T cells we estimate there to be 100\u2013200 epitope-specific cells. After LCMV infection these 100\u2013200 GP33-specific naive CD8 T cells divide &amp;gt;14 times in 1 wk to reach a total of \u223c107 cells. Approximately 5% of these activated GP33-specific effector CD8 T cells survive to generate a memory pool consisting of \u223c5 \u00d7 105 cells. Thus, an acute LCMV infection results in a &amp;gt;1,000-fold increase in precursor frequency of DbGP33-specific CD8 T cells from 2 \u00d7 102 naive cells in uninfected mice to 5 \u00d7 105 memory cells in immunized mice.<\/jats:p>","DOI":"10.1084\/jem.20001021","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T12:48:54Z","timestamp":1027687734000},"page":"657-664","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":485,"title":["Estimating the Precursor Frequency of Naive Antigen-specific CD8 T Cells"],"prefix":"10.1084","volume":"195","author":[{"given":"Joseph N.","family":"Blattman","sequence":"first","affiliation":[{"name":"1Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rustom","family":"Antia","sequence":"additional","affiliation":[{"name":"1Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.D.","family":"Sourdive","sequence":"additional","affiliation":[{"name":"2Unite de Biologie Moleculaire du Gene Institut Pasteur 25, rue du Dr. Roux 75724, Paris, Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochi","family":"Wang","sequence":"additional","affiliation":[{"name":"1Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan M.","family":"Kaech","sequence":"additional","affiliation":[{"name":"1Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaja","family":"Murali-Krishna","sequence":"additional","affiliation":[{"name":"1Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Altman","sequence":"additional","affiliation":[{"name":"1Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafi","family":"Ahmed","sequence":"additional","affiliation":[{"name":"1Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2002,3,4]]},"reference":[{"key":"2023072511250036600_BIB1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0022-1759(99)00136-2","volume":"230","year":"1999","journal-title":"J. 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