{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:35:22Z","timestamp":1791178522717,"version":"4.1.0"},"reference-count":34,"publisher":"Rockefeller University Press","issue":"8","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,4,15]]},"abstract":"<jats:p>The CDR3 regions of T cell receptor (TCR)-\u03b1 and -\u03b2 chains play central roles in the recognition of antigen (Ag)-MHC complex. TCR repertoire is created on the basis of Ag recognition specificity by CDR3s. To analyze the potential spectrum of TCR-\u03b1 and -\u03b2 to exhibit Ag specificity and generate TCR repertoire, we established hundreds of TCR transfectants bearing a single TCR-\u03b1 or -\u03b2 chain derived from a cytotoxic T cell (CTL) clone, RT-1, specific for HIVgp160 peptide, and randomly picked up TCR-\u03b2 or -\u03b1 chains. Surprisingly, one-third of such TCR-\u03b2 containing random CDR3\u03b2 from naive T cells of normal mice could reconstitute the antigen-reactive TCR coupling with RT-1 TCR-\u03b1. A similar dominant function of TCR-\u03b1 in forming Ag-specific TCR, though low-frequency, was obtained for lymphocytic choriomeningitis virus\u2013specific TCR. Subsequently, we generated TCR-\u03b1 and\/or -\u03b2 transgenic (Tg) mice specific for HIVgp160 peptide, and analyzed the TCR repertoire of Ag-specific CTLs. Similar to the results from TCR reconstitution, TCR-\u03b1 Tg generated CTLs with heterogeneous TCR-\u03b2, whereas TCR-\u03b2 Tg-induced CTLs bearing a single TCR-\u03b1. These findings of Ag recognition with minimum involvement of CDR3\u03b2 expand our understanding regarding the flexibility of the spectrum of TCR and suggest a predominant role of TCR-\u03b1 chain in determining the preimmune repertoire of Ag-specific TCR.<\/jats:p>","DOI":"10.1084\/jem.20010809","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:48:54Z","timestamp":1027702134000},"page":"991-1001","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":62,"title":["Predominant Role of T Cell Receptor (TCR)-\u03b1 Chain in Forming Preimmune TCR Repertoire Revealed by Clonal TCR Reconstitution System"],"prefix":"10.1084","volume":"195","author":[{"given":"Tadashi","family":"Yokosuka","sequence":"first","affiliation":[{"name":"1Department of Molecular Genetics, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan"},{"name":"2Department of Thoracic Surgery, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kan","family":"Takase","sequence":"additional","affiliation":[{"name":"1Department of Molecular Genetics, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Misao","family":"Suzuki","sequence":"additional","affiliation":[{"name":"4Center for Animal Resources and Development, Kumamoto University, Kumamoto 860-0811, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohko","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"5Department of Microbiology and Immunology, Nippon Medical School, Tokyo 113-8602, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinsuke","family":"Taki","sequence":"additional","affiliation":[{"name":"1Department of Molecular Genetics, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidemi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"5Department of Microbiology and Immunology, Nippon Medical School, Tokyo 113-8602, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takehiko","family":"Fujisawa","sequence":"additional","affiliation":[{"name":"2Department of Thoracic Surgery, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisashi","family":"Arase","sequence":"additional","affiliation":[{"name":"1Department of Molecular Genetics, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Saito","sequence":"additional","affiliation":[{"name":"1Department of Molecular Genetics, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan"},{"name":"3Cell Signaling Team, RIKEN Research Center for Allergy and Immunology, Kanagawa 230-0045, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2002,4,15]]},"reference":[{"key":"2023072511331967500_BIB1","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1038\/334395a0","volume":"334","year":"1988","journal-title":"Nature."},{"key":"2023072511331967500_BIB2","doi-asserted-by":"crossref","first-page":"10327","DOI":"10.1016\/S0021-9258(18)81620-9","volume":"264","year":"1989","journal-title":"J. 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