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The NF-\u03baB\u2013dependent gene profile comprises chemokines, cytokines, receptors, apoptotic regulators, intracellular signaling molecules, and transcription factors, the majority of which maintain a marker-like expression in HRS cells. Remarkably, we found 17 novel NF-\u03baB target genes. Using chromatin immunoprecipitation we demonstrate that NF-\u03baB is recruited directly to the promoters of several target genes, including signal transducer and activator of transcription (STAT)5a, interleukin-13, and CC chemokine receptor 7. Intriguingly, NF-\u03baB positively regulates STAT5a expression and signaling pathways in HRS cells, and promotes its persistent activation. In fact, STAT5a overexpression was found in most tumor cells of tested patients with classical HD, indicating a critical role for HD. The gene profile underscores a central role of NF-\u03baB in the pathogenesis of HD and potentially of other tumors with constitutive NF-\u03baB activation.<\/jats:p>","DOI":"10.1084\/jem.20020062","type":"journal-article","created":{"date-parts":[[2002,9,3]],"date-time":"2002-09-03T23:17:22Z","timestamp":1031095042000},"page":"605-617","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":227,"title":["Nuclear Factor \u03baB\u2013dependent Gene Expression Profiling of Hodgkin's Disease Tumor Cells, Pathogenetic Significance, and Link to Constitutive Signal Transducer and Activator of Transcription 5a Activity"],"prefix":"10.1084","volume":"196","author":[{"given":"Michael","family":"Hinz","sequence":"first","affiliation":[{"name":"1Max-Delbru\u0308ck Center for Molecular Medicine, 13125 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Lemke","sequence":"additional","affiliation":[{"name":"1Max-Delbru\u0308ck Center for Molecular Medicine, 13125 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Anagnostopoulos","sequence":"additional","affiliation":[{"name":"2Institute for Pathology, Universita\u0308tsklinikum Benjamin Franklin, Free University, 12200 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Hacker","sequence":"additional","affiliation":[{"name":"1Max-Delbru\u0308ck Center for Molecular Medicine, 13125 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Krappmann","sequence":"additional","affiliation":[{"name":"1Max-Delbru\u0308ck Center for Molecular Medicine, 13125 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Mathas","sequence":"additional","affiliation":[{"name":"1Max-Delbru\u0308ck Center for Molecular Medicine, 13125 Berlin, Germany"},{"name":"3Robert-Ro\u0308ssle-Klinik, Universita\u0308tsklinikum Charite\u0301, Humboldt University of Berlin, 13125 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Do\u0308rken","sequence":"additional","affiliation":[{"name":"1Max-Delbru\u0308ck Center for Molecular Medicine, 13125 Berlin, Germany"},{"name":"3Robert-Ro\u0308ssle-Klinik, Universita\u0308tsklinikum Charite\u0301, Humboldt University of Berlin, 13125 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Zenke","sequence":"additional","affiliation":[{"name":"1Max-Delbru\u0308ck Center for Molecular Medicine, 13125 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Stein","sequence":"additional","affiliation":[{"name":"2Institute for Pathology, Universita\u0308tsklinikum Benjamin Franklin, Free University, 12200 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus","family":"Scheidereit","sequence":"additional","affiliation":[{"name":"1Max-Delbru\u0308ck Center for Molecular Medicine, 13125 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2002,8,26]]},"reference":[{"key":"2023072511463738500_BIB1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0092-8674(00)81318-5","volume":"87","year":"1996","journal-title":"Cell."},{"key":"2023072511463738500_BIB2","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1146\/annurev.immunol.14.1.649","volume":"14","year":"1996","journal-title":"Annu. 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