{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:59:28Z","timestamp":1791295168238,"version":"4.1.0"},"reference-count":42,"publisher":"Rockefeller University Press","issue":"9","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,11,4]]},"abstract":"<jats:p>Platelet-endothelial cell adhesion molecule (PECAM)-1 has been implicated in leukocyte migration through the perivascular basement membrane (PBM) though the mechanisms involved are unclear. The present results demonstrate that the ability of \u03b16 integrins to mediate neutrophil migration through the PBM is PECAM-1 dependent, a response associated with PECAM-1\u2013mediated increased expression of \u03b16\u03b21 on transmigrating neutrophils in vivo. An anti-\u03b16 integrins mAb (GoH3) inhibited (78%, P &amp;lt; 0.001) neutrophil migration through interleukin (IL)-1\u03b2\u2013stimulated cremasteric venules, primarily at the level of the PBM, as analyzed by intravital and electron microscopy. In PECAM-1\u2013deficient mice (KO), a reduced level of neutrophil transmigration elicited by IL-1\u03b2 (4-h reaction) was observed in both the cremaster muscle (55% inhibition, P &amp;lt; 0.05) and in the peritoneum (57% inhibition, P &amp;lt; 0.01) but GoH3 had no additional inhibitory effect on these responses. FACS\u00ae analysis of neutrophils demonstrated increased expression of \u03b16\u03b21 on transmigrated peritoneal neutrophils, as compared with blood neutrophils, in wild-type but not KO mice even though neutrophils from both strains of mice exhibited comparable levels of intracellular expression of \u03b16 as observed by immunofluorescent staining and confocal microscopy. Furthermore, mice deficient in either leukocyte or endothelial cell PECAM-1, as developed by bone marrow transplantation, demonstrated a similar level of reduced neutrophil transmigration and expression of \u03b16\u03b21 on transmigrated neutrophils as that detected in KO mice.<\/jats:p>\n                  <jats:p>The results demonstrate a role for PECAM-1 homophilic interaction in neutrophil transmigration and increased expression of \u03b16\u03b21 on the cell surface of transmigrated neutrophils in vivo, a response that could contribute to the mechanism of PECAM-1\u2013mediated neutrophil migration through the PBM.<\/jats:p>","DOI":"10.1084\/jem.20020324","type":"journal-article","created":{"date-parts":[[2002,11,4]],"date-time":"2002-11-04T12:42:44Z","timestamp":1036413764000},"page":"1201-1212","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":193,"title":["PECAM-1 (CD31) Homophilic Interaction Up-Regulates \u03b16\u03b21 on Transmigrated Neutrophils In Vivo and Plays a Functional Role in the Ability of \u03b16 Integrins to Mediate Leukocyte Migration through the Perivascular Basement Membrane"],"prefix":"10.1084","volume":"196","author":[{"given":"John","family":"Dangerfield","sequence":"first","affiliation":[{"name":"1Cardiovascular Medicine Unit, National Heart & Lung Institute, Faculty of Medicine, Imperial College, Hammersmith Hospital, London W12 ONN, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen Y.","family":"Larbi","sequence":"additional","affiliation":[{"name":"1Cardiovascular Medicine Unit, National Heart & Lung Institute, Faculty of Medicine, Imperial College, Hammersmith Hospital, London W12 ONN, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao-Tzu","family":"Huang","sequence":"additional","affiliation":[{"name":"1Cardiovascular Medicine Unit, National Heart & Lung Institute, Faculty of Medicine, Imperial College, Hammersmith Hospital, London W12 ONN, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Dewar","sequence":"additional","affiliation":[{"name":"2National Heart and Lung Institute, Electron Microscopy Unit, Imperial College, Royal Brompton Hospital, London SW3 6NP, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sussan","family":"Nourshargh","sequence":"additional","affiliation":[{"name":"1Cardiovascular Medicine Unit, National Heart & Lung Institute, Faculty of Medicine, Imperial College, Hammersmith Hospital, London W12 ONN, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2002,11,4]]},"reference":[{"key":"2023072511501900700_BIB1","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1182\/blood.V84.7.2068.2068","volume":"84","year":"1994","journal-title":"Blood."},{"key":"2023072511501900700_BIB2","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/1050-1738(94)00028-T","volume":"5","year":"1995","journal-title":"Trends Cardiovasc. 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