{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T22:02:09Z","timestamp":1790373729072,"version":"4.1.0"},"reference-count":30,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,9,2]]},"abstract":"<jats:p>Little is known about the signals that promote early B lineage differentiation from common lymphoid progenitors (CLPs). Using a stromal-free culture system, we show that interleukin (IL)-7 is sufficient to promote the in vitro differentiation of CLPs into B220+ CD19+ B lineage progenitors. Consistent with current models of early B cell development, surface expression of B220 was initiated before CD19 and was accompanied by the loss of T lineage potential. To address whether IL-7 receptor (R) activity is essential for early B lineage development in vivo, we examined the frequencies of CLPs and downstream pre\u2013pro- and pro-B cells in adult mice lacking either the \u03b1 chain or the common gamma chain (\u03b3c) of the IL-7R. The data indicate that although \u03b3c\u2212\/\u2212 mice have normal frequencies of CLPs, both \u03b3c\u2212\/\u2212 and IL-7R\u03b1\u2212\/\u2212 mice lack detectable numbers of all downstream early B lineage precursors, including pre\u2013pro-B cells. These findings challenge previous notions regarding the point in B cell development affected by the loss of IL-7R signaling and suggest that IL-7 plays a key and requisite role during the earliest phases of B cell development.<\/jats:p>","DOI":"10.1084\/jem.20020784","type":"journal-article","created":{"date-parts":[[2002,9,3]],"date-time":"2002-09-03T23:17:22Z","timestamp":1031095042000},"page":"705-711","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":164,"title":["The Earliest Step in B Lineage Differentiation from Common Lymphoid Progenitors Is Critically Dependent upon Interleukin 7"],"prefix":"10.1084","volume":"196","author":[{"given":"Juli P.","family":"Miller","sequence":"first","affiliation":[{"name":"1Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Izon","sequence":"additional","affiliation":[{"name":"1Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"DeMuth","sequence":"additional","affiliation":[{"name":"2Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Gerstein","sequence":"additional","affiliation":[{"name":"3Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avinash","family":"Bhandoola","sequence":"additional","affiliation":[{"name":"1Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Allman","sequence":"additional","affiliation":[{"name":"1Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104"},{"name":"2Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2002,9,2]]},"reference":[{"key":"2023072511490320500_BIB1","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1038\/333571a0","volume":"333","year":"1988","journal-title":"Nature"},{"key":"2023072511490320500_BIB2","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1084\/jem.170.1.333","volume":"170","year":"1989","journal-title":"J. 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