{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:17:32Z","timestamp":1791253052262,"version":"4.1.0"},"reference-count":53,"publisher":"Rockefeller University Press","issue":"11","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,6,2]]},"abstract":"<jats:p>We have used cDNA arrays to investigate gene expression patterns in peripheral blood mononuclear cells from patients with leukemic forms of cutaneous T cell lymphoma, primarily Sezary syndrome (SS). When expression data for patients with high blood tumor burden (Sezary cells &amp;gt;60% of the lymphocytes) and healthy controls are compared by Student's t test, at P &amp;lt; 0.01, we find 385 genes to be differentially expressed. Highly overexpressed genes include Th2 cells\u2013specific transcription factors Gata-3 and Jun B, as well as integrin \u03b21, proteoglycan 2, the RhoB oncogene, and dual specificity phosphatase 1. Highly underexpressed genes include CD26, Stat-4, and the IL-1 receptors. Message for plastin-T, not normally expressed in lymphoid tissue, is detected only in patient samples and may provide a new marker for diagnosis. Using penalized discriminant analysis, we have identified a panel of eight genes that can distinguish SS in patients with as few as 5% circulating tumor cells. This suggests that, even in early disease, Sezary cells produce chemokines and cytokines that induce an expression profile in the peripheral blood distinctive to SS. Finally, we show that using 10 genes, we can identify a class of patients who will succumb within six months of sampling regardless of their tumor burden.<\/jats:p>","DOI":"10.1084\/jem.20021726","type":"journal-article","created":{"date-parts":[[2003,6,4]],"date-time":"2003-06-04T01:43:49Z","timestamp":1054691029000},"page":"1477-1488","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":166,"title":["Classification and Prediction of Survival in Patients with the Leukemic Phase of Cutaneous T Cell Lymphoma"],"prefix":"10.1084","volume":"197","author":[{"given":"Laszlo","family":"Kari","sequence":"first","affiliation":[{"name":"1Molecular Oncology Program, The Wistar Institute, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey","family":"Loboda","sequence":"additional","affiliation":[{"name":"1Molecular Oncology Program, The Wistar Institute, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Nebozhyn","sequence":"additional","affiliation":[{"name":"1Molecular Oncology Program, The Wistar Institute, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain H.","family":"Rook","sequence":"additional","affiliation":[{"name":"2Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric C.","family":"Vonderheid","sequence":"additional","affiliation":[{"name":"3Department of Dermatology, The Johns Hopkins University, Baltimore, MD 21218"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Calen","family":"Nichols","sequence":"additional","affiliation":[{"name":"1Molecular Oncology Program, The Wistar Institute, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dezso","family":"Virok","sequence":"additional","affiliation":[{"name":"1Molecular Oncology Program, The Wistar Institute, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celia","family":"Chang","sequence":"additional","affiliation":[{"name":"1Molecular Oncology Program, The Wistar Institute, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Hwai","family":"Horng","sequence":"additional","affiliation":[{"name":"1Molecular Oncology Program, The Wistar Institute, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Johnston","sequence":"additional","affiliation":[{"name":"1Molecular Oncology Program, The Wistar Institute, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Wysocka","sequence":"additional","affiliation":[{"name":"2Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael K.","family":"Showe","sequence":"additional","affiliation":[{"name":"1Molecular Oncology Program, The Wistar Institute, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise C.","family":"Showe","sequence":"additional","affiliation":[{"name":"1Molecular Oncology Program, The Wistar Institute, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2003,6,2]]},"reference":[{"key":"2023072511524618200_BIB1","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1111\/1523-1747.ep12363017","volume":"107","year":"1996","journal-title":"J. Invest. Dermatol."},{"key":"2023072511524618200_BIB2","first-page":"276","volume":"26","year":"1999","journal-title":"Semin. Oncol."},{"key":"2023072511524618200_BIB3","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1046\/j.1365-2133.2000.03889.x","volume":"143","year":"2000","journal-title":"Br. J. Dermatol."},{"key":"2023072511524618200_BIB4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1749-6632.2001.tb03705.x","volume":"941","year":"2001","journal-title":"Ann. NY Acad. Sci."},{"key":"2023072511524618200_BIB5","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1001\/archderm.1995.01690210033005","volume":"131","year":"1995","journal-title":"Arch. Dermatol."},{"key":"2023072511524618200_BIB6","first-page":"26","volume":"135","year":"1999","journal-title":"Arch. Dermatol."},{"key":"2023072511524618200_BIB7","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1182\/blood.V88.4.1383.bloodjournal8841383","volume":"88","year":"1996","journal-title":"Blood."},{"key":"2023072511524618200_BIB8","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1006\/clin.1994.1170","volume":"73","year":"1994","journal-title":"Clin. Immunol. Immunopathol."},{"key":"2023072511524618200_BIB9","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1001\/archderm.1993.01680250098016","volume":"129","year":"1993","journal-title":"Arch. Dermatol."},{"key":"2023072511524618200_BIB10","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1111\/1523-1747.ep12398454","volume":"103","year":"1994","journal-title":"J. Invest. Dermatol."},{"key":"2023072511524618200_BIB11","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1038\/sj.jidsp.5640188","volume":"4","year":"1999","journal-title":"J. Investig. Dermatol. Symp. Proc."},{"key":"2023072511524618200_BIB12","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1016\/S0190-9622(96)90119-X","volume":"35","year":"1996","journal-title":"J. Am. Acad. Dermatol."},{"key":"2023072511524618200_BIB13","first-page":"73","volume":"23","year":"1995","journal-title":"Annals of Statistics."},{"key":"2023072511524618200_BIB14","first-page":"189","volume":"19","year":"2001","journal-title":"TIBS."},{"key":"2023072511524618200_BIB15","doi-asserted-by":"crossref","first-page":"4073","DOI":"10.4049\/jimmunol.163.7.4073","volume":"163","year":"1999","journal-title":"J. Immunol."},{"key":"2023072511524618200_BIB16","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.4049\/jimmunol.158.3.1085","volume":"158","year":"1997","journal-title":"J. Immunol."},{"key":"2023072511524618200_BIB17","doi-asserted-by":"crossref","first-page":"2648","DOI":"10.4049\/jimmunol.158.6.2648","volume":"158","year":"1997","journal-title":"J. Immunol."},{"key":"2023072511524618200_BIB18","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1006\/clim.1999.4752","volume":"92","year":"1999","journal-title":"Clin. Immunol."},{"key":"2023072511524618200_BIB19","doi-asserted-by":"crossref","first-page":"2559","DOI":"10.1084\/jem.183.6.2559","volume":"183","year":"1996","journal-title":"J. Exp. Med."},{"key":"2023072511524618200_BIB20","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1073\/pnas.87.5.1663","volume":"87","year":"1990","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072511524618200_BIB21","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/1523-1747.ep12611877","volume":"99","year":"1992","journal-title":"J. Invest. Dermatol."},{"key":"2023072511524618200_BIB22","doi-asserted-by":"crossref","first-page":"14863","DOI":"10.1073\/pnas.95.25.14863","volume":"95","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072511524618200_BIB23","doi-asserted-by":"crossref","first-page":"5228","DOI":"10.1074\/jbc.M007542200","volume":"276","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2023072511524618200_BIB24","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1089\/104454999315592","volume":"18","year":"1999","journal-title":"DNA Cell Biol."},{"key":"2023072511524618200_BIB25","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1034\/j.1600-0854.2001.002005303.x","volume":"2","year":"2001","journal-title":"Traffic."},{"key":"2023072511524618200_BIB26","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/S0955-0674(99)80007-6","volume":"11","year":"1999","journal-title":"Curr. Opin. Cell Biol."},{"key":"2023072511524618200_BIB27","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/S0014-5793(97)00317-7","volume":"410","year":"1997","journal-title":"FEBS Lett."},{"key":"2023072511524618200_BIB28","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1089\/107999001459123","volume":"21","year":"2001","journal-title":"J. Interferon Cytokine Res."},{"key":"2023072511524618200_BIB29","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1309\/U1Y6-J4AG-5M4M-7AYV","volume":"115","year":"2001","journal-title":"Am. J. Clin. Pathol."},{"key":"2023072511524618200_BIB30","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1023\/A:1008397323199","volume":"9","year":"1998","journal-title":"Ann. Oncol."},{"key":"2023072511524618200_BIB31"},{"key":"2023072511524618200_BIB32","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1053\/sder.2000.8329","volume":"19","year":"2000","journal-title":"Semin. Cutan. Med. Surg."},{"key":"2023072511524618200_BIB33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0167-9473(01)00046-9","volume":"39","year":"2002","journal-title":"Computational Statistics and Data Analysis."},{"key":"2023072511524618200_BIB34","first-page":"205","volume":"14","year":"2001","journal-title":"Immunity."},{"key":"2023072511524618200_BIB35","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1258\/0007142001903634","volume":"56","year":"2000","journal-title":"Br. Med. Bull."},{"key":"2023072511524618200_BIB36","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1093\/emboj\/18.2.420","volume":"18","year":"1999","journal-title":"EMBO J."},{"key":"2023072511524618200_BIB37","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1182\/blood-2002-08-2434","volume":"101","year":"2003","journal-title":"Blood."},{"key":"2023072511524618200_BIB38","first-page":"1399","volume":"72","year":"2000","journal-title":"Seikagaku."},{"key":"2023072511524618200_BIB39","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/j.1749-6632.2001.tb03706.x","volume":"941","year":"2001","journal-title":"Ann. NY Acad. Sci."},{"key":"2023072511524618200_BIB40","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1002\/(SICI)1096-9896(199604)178:4<385::AID-PATH483>3.0.CO;2-W","volume":"178","year":"1996","journal-title":"J. Pathol."},{"key":"2023072511524618200_BIB41","doi-asserted-by":"crossref","first-page":"3292","DOI":"10.4049\/jimmunol.164.6.3292","volume":"164","year":"2000","journal-title":"J. Immunol."},{"key":"2023072511524618200_BIB42","doi-asserted-by":"crossref","first-page":"713","DOI":"10.4049\/jimmunol.168.2.713","volume":"168","year":"2002","journal-title":"J. Immunol."},{"key":"2023072511524618200_BIB43","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1172\/JCI11517","volume":"107","year":"2001","journal-title":"J. Clin. Invest."},{"key":"2023072511524618200_BIB44","doi-asserted-by":"crossref","first-page":"397","DOI":"10.4049\/jimmunol.165.1.397","volume":"165","year":"2000","journal-title":"J. Immunol."},{"key":"2023072511524618200_BIB45","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S1074-7613(02)00266-2","volume":"16","year":"2002","journal-title":"Immunity."},{"key":"2023072511524618200_BIB46","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1093\/oxfordjournals.jbchem.a123929","volume":"112","year":"1992","journal-title":"J. Biochem. (Tokyo)."},{"key":"2023072511524618200_BIB47","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.1083\/jcb.127.6.1995","volume":"127","year":"1994","journal-title":"J. Cell Biol."},{"key":"2023072511524618200_BIB48","doi-asserted-by":"crossref","first-page":"4659","DOI":"10.4049\/jimmunol.164.9.4659","volume":"164","year":"2000","journal-title":"J. Immunol."},{"key":"2023072511524618200_BIB49","doi-asserted-by":"crossref","first-page":"4446","DOI":"10.4049\/jimmunol.166.7.4446","volume":"166","year":"2001","journal-title":"J. Immunol."},{"key":"2023072511524618200_BIB50","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1097\/00001813-200103000-00001","volume":"12","year":"2001","journal-title":"Anticancer Drugs."},{"key":"2023072511524618200_BIB51","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1038\/35101096","volume":"1","year":"2001","journal-title":"Nat. Rev. Cancer."},{"key":"2023072511524618200_BIB52","doi-asserted-by":"crossref","first-page":"2767","DOI":"10.1517\/13543784.9.12.2767","volume":"9","year":"2000","journal-title":"Expert Opin. Investig. Drugs."},{"key":"2023072511524618200_BIB53"}],"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/197\/11\/1477\/1708984\/jem197111477.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/197\/11\/1477\/1708984\/jem197111477.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T22:53:14Z","timestamp":1704840794000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/197\/11\/1477\/39654\/Classification-and-Prediction-of-Survival-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,6,2]]},"references-count":53,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2003,6,2]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.20021726","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[2003,6,2]]}}}