{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:01:41Z","timestamp":1791291701546,"version":"4.1.0"},"reference-count":18,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,8,4]]},"abstract":"<jats:p>Access to the splenic white pulp is restricted to lymphocytes and dendritic cells. Here we show that movement of molecules from the blood into these confined areas is also limited. Large molecules, such as bovine serum albumin (68 kD), immunoglobulin G (150 kD), and 500 kD dextran are unable to enter the white pulp, whereas smaller blood-borne molecules can directly permeate this compartment. The distribution is restricted to a stromal network that we refer to as the splenic conduit system. The small lumen of the conduit contains collagen fibers and is surrounded in the T cell areas by reticular fibroblasts that express ER-TR7. It also contains the chemokine CCL21. Conversely, in B cell follicles the B cell\u2013attracting chemokine CXCL13 was found to be associated with the conduit and absence of ER-TR7+ fibroblasts. These results show heterogeneity of reticular fibroblasts that enfold the conduit system and suggest that locally produced chemokines are transported through and presented on this reticular network. Therefore, the conduit plays a role in distribution of both blood-borne and locally produced molecules and provides a framework for directing lymphocyte migration and organization of the splenic white pulp.<\/jats:p>","DOI":"10.1084\/jem.20021801","type":"journal-article","created":{"date-parts":[[2003,8,4]],"date-time":"2003-08-04T15:59:23Z","timestamp":1060012763000},"page":"505-512","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":172,"title":["A Conduit System Distributes Chemokines and Small Blood-borne Molecules through the Splenic White Pulp"],"prefix":"10.1084","volume":"198","author":[{"given":"Martijn A.","family":"Nolte","sequence":"first","affiliation":[{"name":"1Department of Molecular Cell Biology, VUMC, 1081 BT Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen A.M.","family":"Belie\u0308n","sequence":"additional","affiliation":[{"name":"2Department of Pathology, VUMC, 1081 BT Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inge","family":"Schadee-Eestermans","sequence":"additional","affiliation":[{"name":"1Department of Molecular Cell Biology, VUMC, 1081 BT Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy","family":"Jansen","sequence":"additional","affiliation":[{"name":"1Department of Molecular Cell Biology, VUMC, 1081 BT Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy W.J.","family":"Unger","sequence":"additional","affiliation":[{"name":"1Department of Molecular Cell Biology, VUMC, 1081 BT Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nico","family":"van Rooijen","sequence":"additional","affiliation":[{"name":"1Department of Molecular Cell Biology, VUMC, 1081 BT Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Kraal","sequence":"additional","affiliation":[{"name":"1Department of Molecular Cell Biology, VUMC, 1081 BT Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reina E.","family":"Mebius","sequence":"additional","affiliation":[{"name":"1Department of Molecular Cell Biology, VUMC, 1081 BT Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2003,8,4]]},"reference":[{"key":"2023072512093621400_BIB1","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1002\/1521-4141(200002)30:2<626::AID-IMMU626>3.0.CO;2-H","volume":"30","year":"2000","journal-title":"Eur. 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