{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:17:04Z","timestamp":1791181024842,"version":"4.1.0"},"reference-count":62,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,3,3]]},"abstract":"<jats:p>Dendritic cells (DCs)**Abbreviations used in this paper: BM, bone marrow; CHS, contact hypersensitivity; cLN, cutaneous lymph node; CRP, C-reactive protein; DC, dendritic cell; DNFB, dinitrofluorobenzene; EGFP, enhanced green fluorescent protein; LC, Langerhans cell; LP, lamina propria; MACS, magnetic-activated cell sorting; mLN, mesenteric lymph node; ODN, oligodeoxynucleotide; PFA, paraformaldehyde; PP, Peyer's patch; TLR, Toll-like receptor; TRITC, tetramethylrhodamine-5-(and-6-)-isothiocyanate. fulfill an important regulatory function at the interface of the innate and adaptive immune system. The thymus and activation-regulated chemokine (TARC\/CCL17) is produced by DCs and facilitates the attraction of activated T cells. Using a fluorescence-based in vivo reporter system, we show that CCL17 expression in mice is found in activated Langerhans cells and mature DCs located in various lymphoid and nonlymphoid organs, and is up-regulated after stimulation with Toll-like receptor ligands. DCs expressing CCL17 belong to the CD11b+CD8\u2212Dec205+ DC subset, including the myeloid-related DCs located in the subepithelial dome of Peyer's patches. CCL17-deficient mice mount diminished T cell\u2013dependent contact hypersensitivity responses and display a deficiency in rejection of allogeneic organ transplants. In contrast to lymphoid organs located at external barriers of the skin and mucosa, CCL17 is not expressed in the spleen, even after systemic microbial challenge or after in vitro stimulation. These findings indicate that CCL17 production is a hallmark of local DC stimulation in peripheral organs but is absent from the spleen as a filter of blood-borne antigens.<\/jats:p>","DOI":"10.1084\/jem.20021859","type":"journal-article","created":{"date-parts":[[2003,3,3]],"date-time":"2003-03-03T18:44:12Z","timestamp":1046717052000},"page":"585-599","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":174,"title":["Compartmentalized Production of CCL17 In Vivo"],"prefix":"10.1084","volume":"197","author":[{"given":"Judith","family":"Alferink","sequence":"first","affiliation":[{"name":"1Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, D-81675 Munich, Germany"},{"name":"2Department of Internal Medicine II, Technical University of Munich, D-81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivo","family":"Lieberam","sequence":"additional","affiliation":[{"name":"4Institute for Genetics, University of Cologne, D-50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Reindl","sequence":"additional","affiliation":[{"name":"1Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, D-81675 Munich, Germany"},{"name":"2Department of Internal Medicine II, Technical University of Munich, D-81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Behrens","sequence":"additional","affiliation":[{"name":"1Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, D-81675 Munich, Germany"},{"name":"2Department of Internal Medicine II, Technical University of Munich, D-81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Wei\u00df","sequence":"additional","affiliation":[{"name":"1Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, D-81675 Munich, Germany"},{"name":"2Department of Internal Medicine II, Technical University of Munich, D-81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"Hu\u0308ser","sequence":"additional","affiliation":[{"name":"1Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, D-81675 Munich, Germany"},{"name":"3Department of Surgery, Technical University of Munich, D-81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Gerauer","sequence":"additional","affiliation":[{"name":"1Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, D-81675 Munich, Germany"},{"name":"3Department of Surgery, Technical University of Munich, D-81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Ross","sequence":"additional","affiliation":[{"name":"5Clinical Research Unit Allergology, Department of Dermatology, Johannes Gutenberg-University of Mainz, D-55101 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika B.","family":"Reske-Kunz","sequence":"additional","affiliation":[{"name":"5Clinical Research Unit Allergology, Department of Dermatology, Johannes Gutenberg-University of Mainz, D-55101 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parviz","family":"Ahmad-Nejad","sequence":"additional","affiliation":[{"name":"1Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, D-81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann","family":"Wagner","sequence":"additional","affiliation":[{"name":"1Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, D-81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irmgard","family":"Fo\u0308rster","sequence":"additional","affiliation":[{"name":"1Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, D-81675 Munich, Germany"},{"name":"2Department of Internal Medicine II, Technical University of Munich, D-81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2003,2,24]]},"reference":[{"key":"2023072511532806600_BIB1","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1146\/annurev.iy.09.040191.001415","volume":"9","year":"1991","journal-title":"Annu. Rev. Immunol."},{"key":"2023072511532806600_BIB2","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1146\/annurev.immunol.18.1.767","volume":"18","year":"2000","journal-title":"Annu. Rev. Immunol."},{"key":"2023072511532806600_BIB3","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1038\/nri746","volume":"2","year":"2002","journal-title":"Nat. Rev. Immunol."},{"key":"2023072511532806600_BIB4","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1038\/ni736","volume":"2","year":"2001","journal-title":"Nat. Immunol."},{"key":"2023072511532806600_BIB5","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1084\/jem.189.3.447","volume":"189","year":"1999","journal-title":"J. Exp. Med."},{"key":"2023072511532806600_BIB6","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1084\/jem.183.4.1287","volume":"183","year":"1996","journal-title":"J. Exp. Med."},{"key":"2023072511532806600_BIB7","first-page":"203","volume":"7","year":"1993","journal-title":"In Vivo."},{"key":"2023072511532806600_BIB8","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1084\/jem.191.3.435","volume":"191","year":"2000","journal-title":"J. Exp. Med."},{"key":"2023072511532806600_BIB9","doi-asserted-by":"crossref","first-page":"2684","DOI":"10.1002\/(SICI)1521-4141(199909)29:09<2684::AID-IMMU2684>3.0.CO;2-Y","volume":"29","year":"1999","journal-title":"Eur. J. Immunol."},{"key":"2023072511532806600_BIB10","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1046\/j.1523-1747.1999.00803.x","volume":"113","year":"1999","journal-title":"J. Invest. Dermatol."},{"key":"2023072511532806600_BIB11","doi-asserted-by":"crossref","first-page":"2934","DOI":"10.1002\/(SICI)1521-4141(199909)29:09<2934::AID-IMMU2934>3.0.CO;2-Q","volume":"29","year":"1999","journal-title":"Eur. J. Immunol."},{"key":"2023072511532806600_BIB12","doi-asserted-by":"crossref","first-page":"2098","DOI":"10.1126\/science.286.5447.2098","volume":"286","year":"1999","journal-title":"Science."},{"key":"2023072511532806600_BIB13","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1093\/intimm\/11.1.81","volume":"11","year":"1999","journal-title":"Int. Immunol."},{"key":"2023072511532806600_BIB14","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/84205","volume":"2","year":"2001","journal-title":"Nat. Immunol."},{"key":"2023072511532806600_BIB15","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/S0167-5699(98)01346-2","volume":"19","year":"1998","journal-title":"Immunol. Today."},{"key":"2023072511532806600_BIB16","doi-asserted-by":"crossref","first-page":"103","DOI":"10.4049\/jimmunol.166.1.103","volume":"166","year":"2001","journal-title":"J. Immunol."},{"key":"2023072511532806600_BIB17","doi-asserted-by":"crossref","first-page":"5027","DOI":"10.4049\/jimmunol.161.9.5027","volume":"161","year":"1998","journal-title":"J. Immunol."},{"key":"2023072511532806600_BIB18","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1038\/23495","volume":"400","year":"1999","journal-title":"Nature."},{"key":"2023072511532806600_BIB19","doi-asserted-by":"crossref","first-page":"15036","DOI":"10.1074\/jbc.272.23.15036","volume":"272","year":"1997","journal-title":"J. Biol. Chem."},{"key":"2023072511532806600_BIB20","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1016\/S0161-5890(02)00013-5","volume":"38","year":"2002","journal-title":"Mol. Immunol."},{"key":"2023072511532806600_BIB21","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1084\/jem.191.10.1755","volume":"191","year":"2000","journal-title":"J. Exp. Med."},{"key":"2023072511532806600_BIB22","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/eji.1830180115","volume":"18","year":"1988","journal-title":"Eur. J. Immunol."},{"key":"2023072511532806600_BIB23","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1084\/jem.188.1.5","volume":"188","year":"1998","journal-title":"J. Exp. Med."},{"key":"2023072511532806600_BIB24","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1093\/intimm\/10.1.61","volume":"10","year":"1998","journal-title":"Int. Immunol."},{"key":"2023072511532806600_BIB25","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1038\/87880","volume":"7","year":"2001","journal-title":"Nat. Med."},{"key":"2023072511532806600_BIB26","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1084\/jem.163.4.981","volume":"163","year":"1986","journal-title":"J. Exp. Med."},{"key":"2023072511532806600_BIB27","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1038\/375151a0","volume":"375","year":"1995","journal-title":"Nature."},{"key":"2023072511532806600_BIB28","doi-asserted-by":"crossref","first-page":"4910","DOI":"10.4049\/jimmunol.165.9.4910","volume":"165","year":"2000","journal-title":"J. Immunol."},{"key":"2023072511532806600_BIB29","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1182\/blood.V96.5.1865","volume":"96","year":"2000","journal-title":"Blood."},{"key":"2023072511532806600_BIB30","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1097\/00007890-199603150-00019","volume":"61","year":"1996","journal-title":"Transplantation."},{"key":"2023072511532806600_BIB31","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1002\/(SICI)1521-4141(199806)28:06<2045::AID-IMMU2045>3.0.CO;2-8","volume":"28","year":"1998","journal-title":"Eur. J. Immunol."},{"key":"2023072511532806600_BIB32","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1006\/cimm.2000.1644","volume":"202","year":"2000","journal-title":"Cell. Immunol."},{"key":"2023072511532806600_BIB33","doi-asserted-by":"crossref","first-page":"4333","DOI":"10.4049\/jimmunol.146.12.4333","volume":"146","year":"1991","journal-title":"J. Immunol."},{"key":"2023072511532806600_BIB34","doi-asserted-by":"crossref","first-page":"741","DOI":"10.4049\/jimmunol.167.2.741","volume":"167","year":"2001","journal-title":"J. Immunol."},{"key":"2023072511532806600_BIB35","first-page":"G1074","volume":"276","year":"1999","journal-title":"Am. J. Physiol."},{"key":"2023072511532806600_BIB36","doi-asserted-by":"crossref","first-page":"4884","DOI":"10.4049\/jimmunol.166.8.4884","volume":"166","year":"2001","journal-title":"J. Immunol."},{"key":"2023072511532806600_BIB37","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1038\/86373","volume":"2","year":"2001","journal-title":"Nat. Immunol."},{"key":"2023072511532806600_BIB38","first-page":"468","volume":"12","year":"2001","journal-title":"Eur. Cytokine Netw."},{"key":"2023072511532806600_BIB39","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1038\/90609","volume":"2","year":"2001","journal-title":"Nat. Immunol."},{"key":"2023072511532806600_BIB40","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1084\/jem.184.4.1413","volume":"184","year":"1996","journal-title":"J. Exp. Med."},{"key":"2023072511532806600_BIB41","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1126\/science.284.5415.819","volume":"284","year":"1999","journal-title":"Science."},{"key":"2023072511532806600_BIB42","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1084\/jem.193.11.1227","volume":"193","year":"2001","journal-title":"J. Exp. Med."},{"key":"2023072511532806600_BIB43","first-page":"673","volume":"3","year":"2002","journal-title":"Nat. Immunol."},{"key":"2023072511532806600_BIB44","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s100240010145","volume":"4","year":"2001","journal-title":"Pediatr. Dev. Pathol."},{"key":"2023072511532806600_BIB45","first-page":"1008","volume":"1","year":"1985","journal-title":"Lancet."},{"key":"2023072511532806600_BIB46","doi-asserted-by":"crossref","first-page":"6296","DOI":"10.4049\/jimmunol.164.12.6296","volume":"164","year":"2000","journal-title":"J. Immunol."},{"key":"2023072511532806600_BIB47","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0074-7696(08)62453-5","volume":"132","year":"1992","journal-title":"Int. Rev. Cytol."},{"key":"2023072511532806600_BIB48","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1084\/jem.20011140","volume":"195","year":"2002","journal-title":"J. Exp. Med."},{"key":"2023072511532806600_BIB49","first-page":"31","volume":"1","year":"2000","journal-title":"Nat. Immunol."},{"key":"2023072511532806600_BIB50","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/S1074-7613(02)00389-8","volume":"17","year":"2002","journal-title":"Immunity."},{"key":"2023072511532806600_BIB51","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.4049\/jimmunol.152.4.1589","volume":"152","year":"1994","journal-title":"J. Immunol."},{"key":"2023072511532806600_BIB52","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1016\/S0002-9440(10)64077-1","volume":"158","year":"2001","journal-title":"Am. J. Pathol."},{"key":"2023072511532806600_BIB53","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1002\/1521-4141(200203)32:3<818::AID-IMMU818>3.0.CO;2-U","volume":"32","year":"2002","journal-title":"Eur. J. Immunol."},{"key":"2023072511532806600_BIB54","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1002\/1521-4141(200105)31:5<1392::AID-IMMU1392>3.0.CO;2-W","volume":"31","year":"2001","journal-title":"Eur. J. Immunol."},{"key":"2023072511532806600_BIB55","doi-asserted-by":"crossref","first-page":"2157","DOI":"10.1084\/jem.185.12.2157","volume":"185","year":"1997","journal-title":"J. Exp. Med."},{"key":"2023072511532806600_BIB56","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/S0952-7915(00)00130-8","volume":"12","year":"2000","journal-title":"Curr. Opin. Immunol."},{"key":"2023072511532806600_BIB57","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1038\/84209","volume":"2","year":"2001","journal-title":"Nat. Immunol."},{"key":"2023072511532806600_BIB58","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1097\/00007890-200110150-00003","volume":"72","year":"2001","journal-title":"Transplantation."},{"key":"2023072511532806600_BIB59","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1097\/00007890-199909270-00001","volume":"68","year":"1999","journal-title":"Transplantation."},{"key":"2023072511532806600_BIB60","doi-asserted-by":"crossref","first-page":"2055","DOI":"10.4049\/jimmunol.166.3.2055","volume":"166","year":"2001","journal-title":"J. Immunol."},{"key":"2023072511532806600_BIB61","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1172\/JCI4619","volume":"102","year":"1998","journal-title":"J. Clin. Invest."},{"key":"2023072511532806600_BIB62","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1084\/jem.194.6.847","volume":"194","year":"2001","journal-title":"J. Exp. Med."}],"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/197\/5\/585\/1709121\/jem1975585.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/197\/5\/585\/1709121\/jem1975585.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T11:54:26Z","timestamp":1690286066000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/197\/5\/585\/39753\/Compartmentalized-Production-of-CCL17-In-Vivo"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,2,24]]},"references-count":62,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,3,3]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.20021859","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[2003,2,24]]}}}