{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T16:51:33Z","timestamp":1761843093158},"reference-count":25,"publisher":"Rockefeller University Press","issue":"7","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,4,7]]},"abstract":"<jats:p>Immunization with vaccinia virus resulted in long-lasting protection against smallpox and was the approach used to eliminate natural smallpox infections worldwide. Due to the concern about the potential use of smallpox virus as a bioweapon, smallpox vaccination is currently being reintroduced. Severe complications from vaccination were associated with congenital or acquired T cell deficiencies, but not with congenital agammaglobulinemia, suggesting the importance of T cell immunity in recovery from infection. In this report, we identified two CD8+ T cell epitopes restricted by the most common human major histocompatibility complex (MHC) class I allele, HLA-A*0201. Both epitopes are highly conserved in vaccinia and variola viruses. The frequency of vaccinia-specific CD8+ T cell responses to these epitopes measured by interferon (IFN)-\u03b3 enzyme-linked immunospot (ELISPOT) assay and HLA\/peptide tetramer staining peaked 2 wk after primary immunization and then declined, but were still detectable 1 to 3 yr after primary immunization. 2 wk after immunization, IFN-\u03b3\u2013producing cells specific to these two epitopes were 14% of total vaccinia virus-specific IFN-\u03b3\u2013producing cells in one donor, 35% in the second donor, and 6% in the third donor. This information will be useful for studies of human T cell memory and for the design and analyses of the immunogenicity of experimental vaccinia vaccines.<\/jats:p>","DOI":"10.1084\/jem.20022222","type":"journal-article","created":{"date-parts":[[2003,4,7]],"date-time":"2003-04-07T20:34:31Z","timestamp":1049747671000},"page":"927-932","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":90,"title":["Quantitation of CD8+ T Cell Responses to Newly Identified HLA-A*0201\u2013restricted T Cell Epitopes Conserved Among Vaccinia and Variola (Smallpox) Viruses"],"prefix":"10.1084","volume":"197","author":[{"given":"Masanori","family":"Terajima","sequence":"first","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Cruz","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory","family":"Raines","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth D.","family":"Kilpatrick","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey S.","family":"Kennedy","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan L.","family":"Rothman","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis A.","family":"Ennis","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2003,3,31]]},"reference":[{"key":"2023072511574731800_BIB1"},{"key":"2023072511574731800_BIB2","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1126\/science.296.5573.1594","volume":"296","year":"2002","journal-title":"Science."},{"key":"2023072511574731800_BIB3","doi-asserted-by":"crossref","first-page":"2274","DOI":"10.1128\/jvi.66.4.2274-2280.1992","volume":"66","year":"1992","journal-title":"J. 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