{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:47:24Z","timestamp":1790801244382,"version":"4.1.0"},"reference-count":54,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,7,21]]},"abstract":"<jats:p>Vaccination with leishmanial Ag and CpG oligodeoxynucleotides (ODN) confers sustained cellular immunity and protection to infectious challenge up to 6 mo after immunization. To define the cellular mechanism by which CpG ODN mediate their adjuvant effects in vivo, the functional capacity of distinct dendritic cell (DC) subsets was assessed in the lymph nodes (LNs) of BALB\/c mice, 36 h after immunization with the leishmanial antigen (LACK) and CpG ODN. After this immunization, there was a striking decrease in the frequency of the CD11c+B220+ plasmacytoid DCs with a proportionate increase in CD11c+CD8\u2212B220\u2212 cells. CD11c+CD8+B220\u2212 cells were the most potent producers of interleukin (IL)-12 p70 and interferon (IFN)-\u03b3, while plasmacytoid DCs were the only subset capable of secreting IFN-\u03b1. In terms of antigen presenting capacity, plasmacytoid DCs were far less efficient compared with the other DC subsets. To certify that DCs were responsible for effective vaccination, we isolated CD11c+ and CD11c\u2212 cells 36 h after immunization and used such cells to elicit protective immunity after adoptive transfer in naive, Leishmania major susceptible BALB\/c mice. CD11c+ cells but not 10-fold higher numbers of CD11c\u2212 cells from such immunized mice mediated protection. Therefore, the combination of LACK antigen and CpG ODN adjuvant leads to the presence of CD11c+ DCs in the draining LN that are capable of vaccinating naive mice in the absence of further antigen or adjuvant.<\/jats:p>","DOI":"10.1084\/jem.20030645","type":"journal-article","created":{"date-parts":[[2003,7,21]],"date-time":"2003-07-21T20:03:35Z","timestamp":1058817815000},"page":"281-291","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":53,"title":["Dendritic Cells Are Responsible for the Capacity of CpG Oligodeoxynucleotides to Act as an Adjuvant for Protective Vaccine Immunity Against <i>Leishmania major<\/i> in Mice"],"prefix":"10.1084","volume":"198","author":[{"given":"Javeed A.","family":"Shah","sequence":"first","affiliation":[{"name":"1Cellular Immunology Section, Vaccine Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A.","family":"Darrah","sequence":"additional","affiliation":[{"name":"1Cellular Immunology Section, Vaccine Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Ambrozak","sequence":"additional","affiliation":[{"name":"2Human Immunology Section, Vaccine Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara N.","family":"Turon","sequence":"additional","affiliation":[{"name":"1Cellular Immunology Section, Vaccine Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Mendez","sequence":"additional","affiliation":[{"name":"3Intracellular Parasite Biology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Kirman","sequence":"additional","affiliation":[{"name":"1Cellular Immunology Section, Vaccine Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-You","family":"Wu","sequence":"additional","affiliation":[{"name":"1Cellular Immunology Section, Vaccine Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Glaichenhaus","sequence":"additional","affiliation":[{"name":"4Centre National de la Recherche Scientifique, UMR 6097, Institute de Pharmacologie Mole\u0301culaire et Cellulaire, 06560 Valbonne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Seder","sequence":"additional","affiliation":[{"name":"1Cellular Immunology Section, Vaccine Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2003,7,21]]},"reference":[{"key":"2023072512055188700_BIB1","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/35021239","volume":"406","year":"2000","journal-title":"Nature."},{"key":"2023072512055188700_BIB2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S1074-7613(00)80533-6","volume":"8","year":"1998","journal-title":"Immunity."},{"key":"2023072512055188700_BIB3","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev.immunol.18.1.451","volume":"18","year":"2000","journal-title":"Annu. Rev. Immunol."},{"key":"2023072512055188700_BIB4","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1146\/annurev.immunol.20.100301.064842","volume":"20","year":"2002","journal-title":"Annu. Rev. Immunol."},{"key":"2023072512055188700_BIB5","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/S0952-7915(02)00343-6","volume":"14","year":"2002","journal-title":"Curr. Opin. Immunol."},{"key":"2023072512055188700_BIB6","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1038\/90609","volume":"2","year":"2001","journal-title":"Nat. Immunol."},{"key":"2023072512055188700_BIB7","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.1073\/pnas.93.7.2879","volume":"93","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072512055188700_BIB8","doi-asserted-by":"crossref","first-page":"3591","DOI":"10.1002\/1521-4141(200012)30:12<3591::AID-IMMU3591>3.0.CO;2-J","volume":"30","year":"2000","journal-title":"Eur. J. Immunol."},{"key":"2023072512055188700_BIB9","doi-asserted-by":"crossref","first-page":"9305","DOI":"10.1073\/pnas.96.16.9305","volume":"96","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072512055188700_BIB10","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1084\/jem.186.10.1623","volume":"186","year":"1997","journal-title":"J. Exp. Med."},{"key":"2023072512055188700_BIB11","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1038\/nm0897-849","volume":"3","year":"1997","journal-title":"Nat. Med."},{"key":"2023072512055188700_BIB12","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1038\/75365","volume":"18","year":"2000","journal-title":"Nat. Biotechnol."},{"key":"2023072512055188700_BIB13","doi-asserted-by":"crossref","first-page":"539","DOI":"10.4049\/jimmunol.165.1.539","volume":"165","year":"2000","journal-title":"J. Immunol."},{"key":"2023072512055188700_BIB14","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1084\/jem.20020147","volume":"195","year":"2002","journal-title":"J. Exp. Med."},{"key":"2023072512055188700_BIB15","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1084\/jem.186.7.1137","volume":"186","year":"1997","journal-title":"J. Exp. Med."},{"key":"2023072512055188700_BIB16","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1084\/jem.186.9.1481","volume":"186","year":"1997","journal-title":"J. Exp. Med."},{"key":"2023072512055188700_BIB17","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1002\/(SICI)1521-4141(199806)28:06<2045::AID-IMMU2045>3.0.CO;2-8","volume":"28","year":"1998","journal-title":"Eur. J. Immunol."},{"key":"2023072512055188700_BIB18","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1006\/smim.2001.0323","volume":"13","year":"2001","journal-title":"Semin. Immunol."},{"key":"2023072512055188700_BIB19","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1146\/annurev.immunol.19.1.47","volume":"19","year":"2001","journal-title":"Annu. Rev. Immunol."},{"key":"2023072512055188700_BIB20","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1084\/jem.189.3.587","volume":"189","year":"1999","journal-title":"J. Exp. Med."},{"key":"2023072512055188700_BIB21","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0092-8674(01)00455-X","volume":"106","year":"2001","journal-title":"Cell."},{"key":"2023072512055188700_BIB22","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1038\/nri746","volume":"2","year":"2002","journal-title":"Nat. Rev. Immunol."},{"key":"2023072512055188700_BIB23","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1126\/science.7725103","volume":"268","year":"1995","journal-title":"Science."},{"key":"2023072512055188700_BIB24","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1126\/science.274.5286.421","volume":"274","year":"1996","journal-title":"Science."},{"key":"2023072512055188700_BIB25","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/S1074-7613(00)80342-8","volume":"6","year":"1997","journal-title":"Immunity."},{"key":"2023072512055188700_BIB26","doi-asserted-by":"crossref","first-page":"8427","DOI":"10.1073\/pnas.160197797","volume":"97","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072512055188700_BIB27","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/S1074-7613(00)00075-3","volume":"13","year":"2000","journal-title":"Immunity."},{"key":"2023072512055188700_BIB28","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1146\/annurev.iy.13.040195.001343","volume":"13","year":"1995","journal-title":"Annu. Rev. Immunol."},{"key":"2023072512055188700_BIB29","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1126\/science.7904381","volume":"263","year":"1994","journal-title":"Science."},{"key":"2023072512055188700_BIB30","doi-asserted-by":"crossref","first-page":"741","DOI":"10.4049\/jimmunol.167.2.741","volume":"167","year":"2001","journal-title":"J. Immunol."},{"key":"2023072512055188700_BIB31","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1038\/ni736","volume":"2","year":"2001","journal-title":"Nat. Immunol."},{"key":"2023072512055188700_BIB32","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1126\/science.284.5421.1835","volume":"284","year":"1999","journal-title":"Science."},{"key":"2023072512055188700_BIB33","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1126\/science.283.5405.1183","volume":"283","year":"1999","journal-title":"Science."},{"key":"2023072512055188700_BIB34","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/S1074-7613(00)00045-5","volume":"13","year":"2000","journal-title":"Immunity."},{"key":"2023072512055188700_BIB35","first-page":"3036","volume":"31","year":"2001","journal-title":"Eur. J. Immunol."},{"key":"2023072512055188700_BIB36"},{"key":"2023072512055188700_BIB37","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1084\/jem.189.12.1981","volume":"189","year":"1999","journal-title":"J. Exp. Med."},{"key":"2023072512055188700_BIB38","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1084\/jem.194.8.1171","volume":"194","year":"2001","journal-title":"J. Exp. Med."},{"key":"2023072512055188700_BIB39","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1084\/jem.20021031","volume":"196","year":"2002","journal-title":"J. Exp. Med."},{"key":"2023072512055188700_BIB40","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1038\/4151043a","volume":"415","year":"2002","journal-title":"Nature."},{"key":"2023072512055188700_BIB41","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1182\/blood.V99.3.999","volume":"99","year":"2002","journal-title":"Blood."},{"key":"2023072512055188700_BIB42","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1146\/annurev.immunol.19.1.23","volume":"19","year":"2001","journal-title":"Annu. Rev. Immunol."},{"key":"2023072512055188700_BIB43","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1126\/science.1071065","volume":"296","year":"2002","journal-title":"Science."},{"key":"2023072512055188700_BIB44","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1084\/jem.189.3.593","volume":"189","year":"1999","journal-title":"J. Exp. Med."},{"key":"2023072512055188700_BIB45"},{"key":"2023072512055188700_BIB46","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1084\/jem.20021091","volume":"197","year":"2003","journal-title":"J. Exp. Med."},{"key":"2023072512055188700_BIB47","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1084\/jem.20021908","volume":"197","year":"2003","journal-title":"J. Exp. Med."},{"key":"2023072512055188700_BIB48"},{"key":"2023072512055188700_BIB49"},{"key":"2023072512055188700_BIB50","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1146\/annurev.immunol.20.100101.151926","volume":"20","year":"2002","journal-title":"Annu. Rev. Immunol."},{"key":"2023072512055188700_BIB51","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1002\/eji.200323797","volume":"33","year":"2003","journal-title":"Eur. J. Immunol."},{"key":"2023072512055188700_BIB52","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1084\/jem.20021522","volume":"197","year":"2003","journal-title":"J. Exp. Med."},{"key":"2023072512055188700_BIB53","doi-asserted-by":"crossref","first-page":"3171","DOI":"10.4049\/jimmunol.170.6.3171","volume":"170","year":"2003","journal-title":"J. Immunol."},{"key":"2023072512055188700_BIB54","doi-asserted-by":"crossref","first-page":"4531","DOI":"10.4049\/jimmunol.168.9.4531","volume":"168","year":"2002","journal-title":"J. Immunol."}],"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/198\/2\/281\/1710712\/jem1982281.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/198\/2\/281\/1710712\/jem1982281.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T12:12:59Z","timestamp":1690287179000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/198\/2\/281\/8475\/Dendritic-Cells-Are-Responsible-for-the-Capacity"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7,21]]},"references-count":54,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,7,21]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.20030645","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[2003,7,21]]}}}