{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:18:56Z","timestamp":1790061536835,"version":"4.0.1"},"reference-count":60,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,2,2]]},"abstract":"<jats:p>Wiskott-Aldrich syndrome protein\u2013interacting protein (WIP) stabilizes actin filaments and is important for immunoreceptor-mediated signal transduction leading to actin cytoskeleton rearrangement in T and B cells. Here we report a role for WIP in signaling pathways downstream of the high affinity receptor for immunoglobulin (Ig)E (Fc\u03f5RI) in mast cells. WIP-deficient bone marrow\u2013derived mast cells (BMMCs) were impaired in their capacity to degranulate and secrete interleukin 6 after Fc\u03f5RI ligation. Calcium mobilization, phosphorylation of Syk, phospholipase C-g2, and c-Jun NH2-terminal kinase were markedly decreased in WIP-deficient BMMCs. WIP was found to associate with Syk after Fc\u03f5RI ligation and to inhibit Syk degradation as evidenced by markedly diminished Syk levels in WIP-deficient BMMCs. WIP-deficient BMMCs exhibited no apparent defect in their subcortical actin network and were normal in their ability to form protrusions when exposed to an IgE-coated surface. However, the kinetics of actin changes and the cell shape changes that follow Fc\u03f5RI signaling were altered in WIP-deficient BMMCs. These results suggest that WIP regulates Fc\u03f5RI-mediated mast cell activation by regulating Syk levels and actin cytoskeleton rearrangement.<\/jats:p>","DOI":"10.1084\/jem.20030652","type":"journal-article","created":{"date-parts":[[2004,2,2]],"date-time":"2004-02-02T22:13:15Z","timestamp":1075759995000},"page":"357-368","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":45,"title":["WIP Regulates Signaling via the High Affinity Receptor for Immunoglobulin E in Mast Cells"],"prefix":"10.1084","volume":"199","author":[{"given":"Alexander","family":"Kettner","sequence":"first","affiliation":[{"name":"1Division of Immunology, Children's Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lalit","family":"Kumar","sequence":"additional","affiliation":[{"name":"1Division of Immunology, Children's Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ine\u0301s M.","family":"Anto\u0301n","sequence":"additional","affiliation":[{"name":"3Department of Clinical and Biological Sciences, University of Turin, 10043 Orbassano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoji","family":"Sasahara","sequence":"additional","affiliation":[{"name":"1Division of Immunology, Children's Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"de la Fuente","sequence":"additional","affiliation":[{"name":"1Division of Immunology, Children's Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vadim I.","family":"Pivniouk","sequence":"additional","affiliation":[{"name":"1Division of Immunology, Children's Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herve\u0301","family":"Falet","sequence":"additional","affiliation":[{"name":"2Division of Hematology, Brigham and Women's Hospital, Department of Pediatrics and Department of Medicine, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John H.","family":"Hartwig","sequence":"additional","affiliation":[{"name":"2Division of Hematology, Brigham and Women's Hospital, Department of Pediatrics and Department of Medicine, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raif S.","family":"Geha","sequence":"additional","affiliation":[{"name":"1Division of Immunology, Children's Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2004,2,2]]},"reference":[{"key":"2023072512261074100_BIB1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/0092-8674(94)90528-2","volume":"78","year":"1994","journal-title":"Cell."},{"key":"2023072512261074100_BIB2","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1182\/blood.V68.6.1329.1329","volume":"68","year":"1986","journal-title":"Blood."},{"key":"2023072512261074100_BIB3","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1002\/(SICI)1521-4141(199810)28:10<3259::AID-IMMU3259>3.0.CO;2-B","volume":"28","year":"1998","journal-title":"Eur. 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