{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T21:22:09Z","timestamp":1790976129611,"version":"4.1.0"},"reference-count":40,"publisher":"Rockefeller University Press","issue":"12","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,12,15]]},"abstract":"<jats:p>Mammalian SWI\u2013SNF-related complexes use brahma-related gene 1 (Brg1) as a catalytic subunit to remodel nucleosomes and regulate transcription. Recent biochemical data has linked Brg1 function to genes important for T lymphocyte differentiation. To investigate the role of SWI\u2013SNF-related complexes in this lineage, we ablated Brg1 function in T lymphocytes. T cell\u2013specific Brg1-deficient mice showed profound thymic abnormalities, CD4 derepression at the double negative (DN; CD4\u2212 CD8\u2212) stage, and a developmental block at the DN to double positive (CD4+ CD8+) transition. 5\u2032-bromo-2\u2032-deoxyuridine incorporation and annexin V staining establish a role for Brg1 complexes in the regulation of thymocyte cell proliferation and survival. This Brg1-dependent cell survival is specific for developing thymocytes as indicated by the presence of Brg1-deficient mature T lymphocytes that have escaped the developmental block in the thymus. However, reductions in peripheral T cell populations lead to immunodeficiency and compromised health of mutant mice. These results highlight the importance of chromatin-remodeling complexes at different stages in the development of a mammalian cell lineage.<\/jats:p>","DOI":"10.1084\/jem.20030714","type":"journal-article","created":{"date-parts":[[2003,12,15]],"date-time":"2003-12-15T21:02:51Z","timestamp":1071522171000},"page":"1937-1949","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":126,"title":["The Role of <i>Brg1<\/i>, a Catalytic Subunit of Mammalian Chromatin-remodeling Complexes, in T Cell Development"],"prefix":"10.1084","volume":"198","author":[{"given":"Thomas C.","family":"Gebuhr","sequence":"first","affiliation":[{"name":"1Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grigoriy I.","family":"Kovalev","sequence":"additional","affiliation":[{"name":"2Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"},{"name":"4Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott","family":"Bultman","sequence":"additional","affiliation":[{"name":"1Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"Godfrey","sequence":"additional","affiliation":[{"name":"3Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lishan","family":"Su","sequence":"additional","affiliation":[{"name":"2Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"},{"name":"4Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry","family":"Magnuson","sequence":"additional","affiliation":[{"name":"1Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"},{"name":"4Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"},{"name":"5Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2003,12,15]]},"reference":[{"key":"2023072822213961900_BIB1","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1146\/annurev.immunol.17.1.149","volume":"17","year":"1999","journal-title":"Annu. 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