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Our results show that the kinetics of degranulation, assessed by a novel flow cytometric based assay, were identical in effector and both subsets of memory CD8+ T cells, but absent in naive CD8+ T cells. However, immediate cytolytic activity was most pronounced in effector T cells, low in effector memory T cells, and absent in central memory T cells, correlating with the respective levels of cytolytic effector molecules present in lytic granules. These results indicate that an inherent program of degranulation is a feature of antigen-experienced cells as opposed to naive CD8+ T cells and that the ability of CD8+ T cells to induce target cell apoptosis\/death is dependent on granule protein content rather than on the act of degranulation itself. Furthermore, these results provide a potential mechanism by which central memory CD8+ T cell\u2013mediated death of antigen-presenting cells within the lymph node is avoided.<\/jats:p>","DOI":"10.1084\/jem.20031799","type":"journal-article","created":{"date-parts":[[2004,3,29]],"date-time":"2004-03-29T20:54:02Z","timestamp":1080593642000},"page":"925-936","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":208,"title":["Immediate Cytotoxicity But Not Degranulation Distinguishes Effector and Memory Subsets of CD8+ T Cells"],"prefix":"10.1084","volume":"199","author":[{"given":"Petra","family":"Wolint","sequence":"first","affiliation":[{"name":"1Institute for Microbiology, Eidgeno\u0308ssische Technische Hochschule Zu\u0308rich, 8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Betts","sequence":"additional","affiliation":[{"name":"2Laboratory of Immunology, Vaccine Research Center\/National Institute of Allergy and Infectious Diseases\/ National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Koup","sequence":"additional","affiliation":[{"name":"2Laboratory of Immunology, Vaccine Research Center\/National Institute of Allergy and Infectious Diseases\/ National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Oxenius","sequence":"additional","affiliation":[{"name":"1Institute for Microbiology, Eidgeno\u0308ssische Technische Hochschule Zu\u0308rich, 8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2004,3,29]]},"reference":[{"key":"2023072512330087300_BIB1","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/87720","volume":"2","year":"2001","journal-title":"Nat. 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