{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:06:27Z","timestamp":1791219987905,"version":"4.1.0"},"reference-count":31,"publisher":"Rockefeller University Press","issue":"11","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,12,6]]},"abstract":"<jats:p>Marginal zones (MZs) are microdomains in the spleen that contain various types of immune cells, including MZ B cells, MOMA1+ metallophilic macrophages, and mucosal addressin cell adhesion molecule 1 (MAdCAM-1)+ endothelial cells. MAdCAM-1+ and MOMA1+ cells line the sinus, that separates MZs from splenic follicles. Here we show that a receptor for the lysophospholipid sphingosine-1-phosphate (S1P), S1P3, is required for normal numbers of splenic immature and MZ B cells, and for S1P-induced chemotaxis of MZ B cells. S1P3 is also essential for proper alignment of MOMA1+ macrophages and MAdCAM-1+ endothelial cells along the marginal sinus. The lack of cohesion of the marginal sinus in S1P3\u2212\/\u2212 mice affects MZ B cell functions, as wild-type (WT) MZ B cells migrate more into S1P3\u2212\/\u2212 follicles than into WT follicles after treatment with lipopolysaccharide. Additionally, short-term homing experiments demonstrate that WT MZ B cells home to the S1P3\u2212\/\u2212 spleen in increased numbers, suggesting a role for the marginal sinus in regulating MZ B cells numbers. Moreover, S1P3\u2212\/\u2212 mice are defective in mounting immune responses to thymus-independent antigen type 2 due to defects in radiation-resistant cells in the spleen. These data identify lysophospholipids and the S1P3 receptor as essential regulators of the MZ sinus and its role as a barrier to the follicle.<\/jats:p>","DOI":"10.1084\/jem.20041483","type":"journal-article","created":{"date-parts":[[2004,12,6]],"date-time":"2004-12-06T17:42:33Z","timestamp":1102354953000},"page":"1491-1501","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":65,"title":["The Sphingosine-1-Phosphate (S1P) Lysophospholipid Receptor S1P3 Regulates MAdCAM-1+ Endothelial Cells in Splenic Marginal Sinus Organization"],"prefix":"10.1084","volume":"200","author":[{"given":"Irute","family":"Girkontaite","sequence":"first","affiliation":[{"name":"1Department of Physiological Chemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vadim","family":"Sakk","sequence":"additional","affiliation":[{"name":"1Department of Physiological Chemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Wagner","sequence":"additional","affiliation":[{"name":"2Department of Gastroenterology and Endocrinology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tilman","family":"Borggrefe","sequence":"additional","affiliation":[{"name":"3Department of Immmunology, University of Ulm, D-89069 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerry","family":"Tedford","sequence":"additional","affiliation":[{"name":"1Department of Physiological Chemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerold","family":"Chun","sequence":"additional","affiliation":[{"name":"4Department of Molecular Biology, Helen L. 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