{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T04:01:39Z","timestamp":1792123299060,"version":"4.4.0"},"reference-count":29,"publisher":"Rockefeller University Press","issue":"7","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,7,10]]},"abstract":"<jats:p>Abnormalities in CD4+CD25+Foxp3+ regulatory T (T reg) cells have been implicated in susceptibility to allergic, autoimmune, and immunoinflammatory conditions. However, phenotypic and functional assessment of human T reg cells has been hampered by difficulty in distinguishing between CD25-expressing activated and regulatory T cells. Here, we show that expression of CD127, the \u03b1 chain of the interleukin-7 receptor, allows an unambiguous flow cytometry\u2013based distinction to be made between CD127lo T reg cells and CD127hi conventional T cells within the CD25+CD45RO+RA\u2212 effector\/memory and CD45RA+RO\u2212 naive compartments in peripheral blood and lymph node. In healthy volunteers, peripheral blood CD25+CD127lo cells comprised 6.35 \u00b1 0.26% of CD4+ T cells, of which 2.05 \u00b1 0.14% expressed the naive subset marker CD45RA. Expression of FoxP3 protein and the CD127lo phenotype were highly correlated within the CD4+CD25+ population. Moreover, both effector\/memory and naive CD25+CD127lo cells manifested suppressive activity in vitro, whereas CD25+CD127hi cells did not. Cell surface expression of CD127 therefore allows accurate estimation of T reg cell numbers and isolation of pure populations for in vitro studies and should contribute to our understanding of regulatory abnormalities in immunopathic diseases.<\/jats:p>","DOI":"10.1084\/jem.20060468","type":"journal-article","created":{"date-parts":[[2006,7,5]],"date-time":"2006-07-05T17:28:26Z","timestamp":1152120506000},"page":"1693-1700","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":1290,"title":["Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells"],"prefix":"10.1084","volume":"203","author":[{"given":"Nabila","family":"Seddiki","sequence":"first","affiliation":[{"name":"1Centenary Institute of Cancer Medicine and Cell Biology, Faculty of Medicine, University of Sydney, Newtown, NSW 2042, Australia"},{"name":"2Centre for Immunology, St. Vincents Hospital, Darlinghurst, NSW 2010, Australia"},{"name":"3National Centre in HIV Epidemiology and Clinical Research, University of NSW, Kensington, NSW 2052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Santner-Nanan","sequence":"additional","affiliation":[{"name":"4Department of Paediatrics, University of Sydney, Western Clinical School, Penrith, NSW 2751, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff","family":"Martinson","sequence":"additional","affiliation":[{"name":"5Department of Immunology\/Microbiology, Rush University Medical Center, Chicago, IL 60612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Zaunders","sequence":"additional","affiliation":[{"name":"2Centre for Immunology, St. Vincents Hospital, Darlinghurst, NSW 2010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Sasson","sequence":"additional","affiliation":[{"name":"2Centre for Immunology, St. Vincents Hospital, Darlinghurst, NSW 2010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Landay","sequence":"additional","affiliation":[{"name":"5Department of Immunology\/Microbiology, Rush University Medical Center, Chicago, IL 60612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Solomon","sequence":"additional","affiliation":[{"name":"6Department of Gastroenterology, The Royal Prince Alfred Hospital, Camperdown, NSW 2050, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warwick","family":"Selby","sequence":"additional","affiliation":[{"name":"6Department of Gastroenterology, The Royal Prince Alfred Hospital, Camperdown, NSW 2050, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen I.","family":"Alexander","sequence":"additional","affiliation":[{"name":"7The Children's Hospital at Westmead, NSW 2145, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph","family":"Nanan","sequence":"additional","affiliation":[{"name":"4Department of Paediatrics, University of Sydney, Western Clinical School, Penrith, NSW 2751, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Kelleher","sequence":"additional","affiliation":[{"name":"2Centre for Immunology, St. Vincents Hospital, Darlinghurst, NSW 2010, Australia"},{"name":"3National Centre in HIV Epidemiology and Clinical Research, University of NSW, Kensington, NSW 2052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara Fazekas","family":"de St. Groth","sequence":"additional","affiliation":[{"name":"1Centenary Institute of Cancer Medicine and Cell Biology, Faculty of Medicine, University of Sydney, Newtown, NSW 2042, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2006,7,3]]},"reference":[{"key":"2023072515105131400_BIB1","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1146\/annurev.immunol.21.120601.141122","volume":"22","year":"2004","journal-title":"Annu. 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