{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:13:15Z","timestamp":1792804395897,"version":"4.5.0"},"reference-count":57,"publisher":"Rockefeller University Press","issue":"8","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,8,7]]},"abstract":"<jats:p>Neisseria meningitidis is a commensal bacterium of the human nasopharynx. Occasionally, this bacterium reaches the bloodstream and causes meningitis after crossing the blood\u2013brain barrier by an unknown mechanism. An immunohistological study of a meningococcal sepsis case revealed that neisserial adhesion was restricted to capillaries located in low blood flow regions in the infected organs. This study led to the hypothesis that drag forces encountered by the meningococcus in the bloodstream determine its attachment site in vessels. We therefore investigated the ability of N. meningitidis to bind to endothelial cells in the presence of liquid flow mimicking the bloodstream with a laminar flow chamber. Strikingly, average blood flows reported for various organs strongly inhibited initial adhesion. As cerebral microcirculation is known to be highly heterogeneous, cerebral blood velocity was investigated at the level of individual vessels using intravital imaging of rat brain. In agreement with the histological study, shear stress levels compatible with meningococcal adhesion were only observed in capillaries, which exhibited transient reductions in flow. The flow chamber assay revealed that, after initial attachment, bacteria resisted high blood velocities and even multiplied, forming microcolonies resembling those observed in the septicemia case. These results argue that the combined mechanical properties of neisserial adhesion and blood microcirculation target meningococci to transiently underperfused cerebral capillaries and thus determine disease development.<\/jats:p>","DOI":"10.1084\/jem.20060482","type":"journal-article","created":{"date-parts":[[2006,7,24]],"date-time":"2006-07-24T20:25:16Z","timestamp":1153772716000},"page":"1939-1950","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":182,"title":["Cerebral microcirculation shear stress levels determine\n                    <i>Neisseria meningitidis<\/i>\n                    attachment sites along the blood\u2013brain barrier"],"prefix":"10.1084","volume":"203","author":[{"given":"Emilie","family":"Mairey","sequence":"first","affiliation":[{"name":"1Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM), U570 and"},{"name":"3Universite\u0301 Paris Descartes, Faculte\u0301 de Me\u0301decine Rene\u0301 Descartes, UMR-S570, Paris, F-75006 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Auguste","family":"Genovesio","sequence":"additional","affiliation":[{"name":"4Centre National de la Recherche Scientifique, URA2582 and"},{"name":"5Institut Pasteur, Quantitative Image Analysis Unit, Paris, F-75724 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Donnadieu","sequence":"additional","affiliation":[{"name":"6INSERM, U567 and"},{"name":"7Institut Cochin, De\u0301partement de biologie cellulaire, Paris, F-75014 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Bernard","sequence":"additional","affiliation":[{"name":"1Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM), U570 and"},{"name":"3Universite\u0301 Paris Descartes, Faculte\u0301 de Me\u0301decine Rene\u0301 Descartes, UMR-S570, Paris, F-75006 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Jaubert","sequence":"additional","affiliation":[{"name":"2AP-HP, Ho\u0302pital Necker-Enfants Malades, Paris, F-75015 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Pinard","sequence":"additional","affiliation":[{"name":"8INSERM, U689 and"},{"name":"9Universite\u0301 Denis Diderot, Faculte\u0301 de me\u0301decine Lariboisie\u0300re-St. Louis, Paris, F-75010 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Seylaz","sequence":"additional","affiliation":[{"name":"8INSERM, U689 and"},{"name":"9Universite\u0301 Denis Diderot, Faculte\u0301 de me\u0301decine Lariboisie\u0300re-St. Louis, Paris, F-75010 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Christophe","family":"Olivo-Marin","sequence":"additional","affiliation":[{"name":"4Centre National de la Recherche Scientifique, URA2582 and"},{"name":"5Institut Pasteur, Quantitative Image Analysis Unit, Paris, F-75724 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Nassif","sequence":"additional","affiliation":[{"name":"1Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM), U570 and"},{"name":"2AP-HP, Ho\u0302pital Necker-Enfants Malades, Paris, F-75015 France"},{"name":"3Universite\u0301 Paris Descartes, Faculte\u0301 de Me\u0301decine Rene\u0301 Descartes, UMR-S570, Paris, F-75006 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Dume\u0301nil","sequence":"additional","affiliation":[{"name":"1Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM), U570 and"},{"name":"3Universite\u0301 Paris Descartes, Faculte\u0301 de Me\u0301decine Rene\u0301 Descartes, UMR-S570, Paris, F-75006 France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2006,7,24]]},"reference":[{"key":"2023072515062121700_BIB1","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1128\/CMR.13.1.144","volume":"13","year":"2000","journal-title":"Clin. 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