{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:31:59Z","timestamp":1791379919361,"version":"4.3.3"},"reference-count":35,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,2,19]]},"abstract":"<jats:p>Although the immune system evolved to fight infections, it may also attack and destroy solid tumors. In most cases, tumor rejection is initiated by CD8+ cytotoxic T lymphocytes (CTLs), which infiltrate solid tumors, recognize tumor antigens, and kill tumor cells. We use a combination of two-photon intravital microscopy and immunofluorescence on ordered sequential sections to analyze the infiltration and destruction of solid tumors by CTLs. We show that in the periphery of a thymoma growing subcutaneously, activated CTLs migrate with high instantaneous velocities. The CTLs arrest in close contact to tumor cells expressing their cognate antigen. In regions where most tumor cells are dead, CTLs resume migration, sometimes following collagen fibers or blood vessels. CTLs migrating along blood vessels preferentially adopt an elongated morphology. CTLs also infiltrate tumors in depth, but only when the tumor cells express the cognate CTL antigen. In tumors that do not express the cognate antigen, CTL infiltration is restricted to peripheral regions, and lymphocytes neither stop moving nor kill tumor cells. Antigen expression by tumor cells therefore determines both CTL motility within the tumor and profound tumor infiltration.<\/jats:p>","DOI":"10.1084\/jem.20061890","type":"journal-article","created":{"date-parts":[[2007,2,2]],"date-time":"2007-02-02T17:15:18Z","timestamp":1170436518000},"page":"345-356","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":367,"title":["In vivo imaging of cytotoxic T cell infiltration and elimination of a solid tumor"],"prefix":"10.1084","volume":"204","author":[{"given":"Alexandre","family":"Boissonnas","sequence":"first","affiliation":[{"name":"1Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U653, Immunite\u0301 et Cancer, Pavillon Pasteur, Institut Curie, F-75245 Paris Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Fetler","sequence":"additional","affiliation":[{"name":"2Centre National de la Recherche Scientifique UMR 168, Laboratoire de Physico-Chimie Curie, Institut Curie, F-75245 Paris Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid S.","family":"Zeelenberg","sequence":"additional","affiliation":[{"name":"1Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U653, Immunite\u0301 et Cancer, Pavillon Pasteur, Institut Curie, F-75245 Paris Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ste\u0301phanie","family":"Hugues","sequence":"additional","affiliation":[{"name":"1Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U653, Immunite\u0301 et Cancer, Pavillon Pasteur, Institut Curie, F-75245 Paris Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Amigorena","sequence":"additional","affiliation":[{"name":"1Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U653, Immunite\u0301 et Cancer, Pavillon Pasteur, Institut Curie, F-75245 Paris Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2007,1,29]]},"reference":[{"key":"2023072515552944400_BIB1","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1146\/annurev.cellbio.21.122303.133159","volume":"21","year":"2005","journal-title":"Annu. 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