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In the bone marrow of B-S1pr1KO mice, immature B cells in contact with the vascular compartment displayed increased apoptosis as compared with control mice. Forced expression of CD69, a negative regulator of S1P1 receptor expression, in developing bone marrow B cells also reduced the number of immature B cells in the blood. Attenuation of CXCR4 signaling, which is required for the proper retention of developing B cells in bone marrow, did not release immature B cells into the blood of B-S1pr1KO mice as effectively as in control mice. Our results indicate that the S1P1 receptor provides a signal necessary for the efficient transfer of newly generated immature B cells from the bone marrow to the blood.<\/jats:p>","DOI":"10.1084\/jem.20092210","type":"journal-article","created":{"date-parts":[[2010,4,19]],"date-time":"2010-04-19T23:30:01Z","timestamp":1271719801000},"page":"1113-1124","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":168,"title":["S1P1 receptor directs the release of immature B cells from bone marrow into blood"],"prefix":"10.1084","volume":"207","author":[{"given":"Maria L.","family":"Allende","sequence":"first","affiliation":[{"name":"Genetics of Development and Disease Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina","family":"Tuymetova","sequence":"additional","affiliation":[{"name":"Genetics of Development and Disease Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bridgin G.","family":"Lee","sequence":"additional","affiliation":[{"name":"Genetics of Development and Disease Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana","family":"Bonifacino","sequence":"additional","affiliation":[{"name":"Genetics of Development and Disease Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Ping","family":"Wu","sequence":"additional","affiliation":[{"name":"Genetics of Development and Disease Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard L.","family":"Proia","sequence":"additional","affiliation":[{"name":"Genetics of Development and Disease Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2010,4,19]]},"reference":[{"key":"2023072601405822300_bib1","doi-asserted-by":"publisher","first-page":"3665","DOI":"10.1182\/blood-2003-02-0460","article-title":"G-protein-coupled receptor S1P1 acts within endothelial cells to regulate vascular maturation","volume":"102","author":"Allende","year":"2003","journal-title":"Blood."},{"key":"2023072601405822300_bib2","doi-asserted-by":"publisher","first-page":"15396","DOI":"10.1074\/jbc.M314291200","article-title":"Expression of the sphingosine 1-phosphate receptor, S1P1, on T-cells controls thymic emigration","volume":"279","author":"Allende","year":"2004","journal-title":"J. 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