{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T05:08:38Z","timestamp":1791954518157,"version":"4.3.4"},"reference-count":30,"publisher":"Rockefeller University Press","issue":"7","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,7,5]]},"abstract":"<jats:p>The process of erythropoiesis must be efficient and robust to supply the organism with red bloods cells both under condition of homeostasis and stress. The microRNA (miRNA) pathway was recently shown to regulate erythroid development. Here, we show that expression of the locus encoding miR-144 and miR-451 is strictly dependent on Argonaute 2 and is required for erythroid homeostasis. Mice deficient for the miR-144\/451 cluster display a cell autonomous impairment of late erythroblast maturation, resulting in erythroid hyperplasia, splenomegaly, and a mild anemia. Analysis of gene expression profiles from wild-type and miR-144\/451\u2013deficient erythroblasts revealed that the miR-144\/451 cluster acts as a \u201ctuner\u201d of gene expression, influencing the expression of many genes. MiR-451 imparts a greater impact on target gene expression than miR-144. Accordingly, mice deficient in miR-451 alone exhibited a phenotype indistinguishable from miR-144\/451\u2013deficient mice. Thus, the miR-144\/451 cluster tunes gene expression to impart a robustness to erythropoiesis that is critical under conditions of stress.<\/jats:p>","DOI":"10.1084\/jem.20100458","type":"journal-article","created":{"date-parts":[[2010,5,31]],"date-time":"2010-05-31T20:54:27Z","timestamp":1275339267000},"page":"1351-1358","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":291,"title":["The miR-144\/451 locus is required for erythroid homeostasis"],"prefix":"10.1084","volume":"207","author":[{"given":"Kasper D.","family":"Rasmussen","sequence":"first","affiliation":[{"name":"European Molecular Biology Laboratory, Mouse Biology Unit, Monterotondo Scalo, 00015, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Simmini","sequence":"additional","affiliation":[{"name":"European Molecular Biology Laboratory, Mouse Biology Unit, Monterotondo Scalo, 00015, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cei","family":"Abreu-Goodger","sequence":"additional","affiliation":[{"name":"European Bioinformatics Institute, Hinxton, Cambridge, CB10 1SD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nenad","family":"Bartonicek","sequence":"additional","affiliation":[{"name":"European Bioinformatics Institute, Hinxton, Cambridge, CB10 1SD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Di Giacomo","sequence":"additional","affiliation":[{"name":"European Molecular Biology Laboratory, Mouse Biology Unit, Monterotondo Scalo, 00015, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Bilbao-Cortes","sequence":"additional","affiliation":[{"name":"European Molecular Biology Laboratory, Mouse Biology Unit, Monterotondo Scalo, 00015, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rastislav","family":"Horos","sequence":"additional","affiliation":[{"name":"Department of Hematology, Erasmus MC, 3015 GE Rotterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marieke","family":"Von Lindern","sequence":"additional","affiliation":[{"name":"Department of Hematology, Erasmus MC, 3015 GE Rotterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton J.","family":"Enright","sequence":"additional","affiliation":[{"name":"European Bioinformatics Institute, Hinxton, Cambridge, CB10 1SD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00f3nal","family":"O\u2019Carroll","sequence":"additional","affiliation":[{"name":"European Molecular Biology Laboratory, Mouse Biology Unit, Monterotondo Scalo, 00015, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2010,5,31]]},"reference":[{"key":"2020100312001022000_bib1","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1038\/nature07242","article-title":"The impact of microRNAs on protein output","volume":"455","author":"Baek","year":"2008","journal-title":"Nature."},{"key":"2020100312001022000_bib2","doi-asserted-by":"publisher","first-page":"2996","DOI":"10.1101\/gad.13.22.2996","article-title":"The glucocorticoid receptor is required for stress erythropoiesis","volume":"13","author":"Bauer","year":"1999","journal-title":"Genes Dev."},{"key":"2020100312001022000_bib3","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1182\/blood.V88.1.75.75","article-title":"Interaction of stem cell factor and its receptor c-kit mediates lodgment and acute expansion of hematopoietic cells in the murine spleen","volume":"88","author":"Broudy","year":"1996","journal-title":"Blood."},{"key":"2020100312001022000_bib4","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1146\/annurev.cellbio.23.090506.123406","article-title":"microRNA functions","volume":"23","author":"Bushati","year":"2007","journal-title":"Annu. 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