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Accordingly, antibody produced by the polyclonal pool of MBC neutralized WT and variant viruses equivalently. Remarkably, we also identified MBC clones that recognized the mutant epitope better than the WT protein, despite never having been exposed to the variant virus. The ability of MBCs to respond to variant viruses in vivo was confirmed by experiments in which MBCs were adoptively transferred or depleted before secondary challenge. Our data demonstrate that class-switched MBC can respond to variants of the original pathogen that escape neutralization of antibody produced by LLPC without a requirement for accumulating additional somatic mutations.<\/jats:p>","DOI":"10.1084\/jem.20110740","type":"journal-article","created":{"date-parts":[[2011,12,12]],"date-time":"2011-12-12T23:53:04Z","timestamp":1323733984000},"page":"2599-2606","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":264,"title":["Memory B cells, but not long-lived plasma cells, possess antigen specificities for viral escape mutants"],"prefix":"10.1084","volume":"208","author":[{"given":"Whitney E.","family":"Purtha","sequence":"first","affiliation":[{"name":"Department of Pathology and Immunology 1 , 2 , and 3"},{"name":"Department of Medicine 1 , 2 , and 3"},{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas F.","family":"Tedder","sequence":"additional","affiliation":[{"name":"Department of Immunology, Duke University Medical Center, Durham, NC 27710 4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syd","family":"Johnson","sequence":"additional","affiliation":[{"name":"MacroGenics, Inc., Rockville, MD 20850 5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepta","family":"Bhattacharya","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology 1 , 2 , and 3"},{"name":"Department of Medicine 1 , 2 , and 3"},{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael S.","family":"Diamond","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology 1 , 2 , and 3"},{"name":"Department of Medicine 1 , 2 , and 3"},{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110 1 , 2 , and 3"},{"name":"Department of Pathology and Immunology 1 , 2 , and 3"},{"name":"Department of Medicine 1 , 2 , and 3"},{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110 1 , 2 , and 3"},{"name":"Department of Pathology and Immunology 1 , 2 , and 3"},{"name":"Department of Medicine 1 , 2 , and 3"},{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2011,12,12]]},"reference":[{"key":"2023072918004489900_bib1","doi-asserted-by":"publisher","first-page":"4802","DOI":"10.1073\/pnas.0800555105","article-title":"Maintenance of the plasma cell pool is independent of memory B cells","volume":"105","author":"Ahuja","year":"2008","journal-title":"Proc. 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