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Physiological mechanisms that influence CSR to C\u03b31 versus C\u03b5 are incompletely understood. In this study, we report a role for B cell developmental maturity in IgE CSR. Based in part on a novel flow cytometric IgE CSR assay, we show that immature B cells preferentially switch to IgE versus IgG1 through a mechanism involving increased direct CSR from C\u03bc to C\u03b5. Our findings suggest that IgE dysregulation in certain immunodeficiencies may be related to impaired B cell maturation.<\/jats:p>","DOI":"10.1084\/jem.20111155","type":"journal-article","created":{"date-parts":[[2011,12,5]],"date-time":"2011-12-05T22:10:57Z","timestamp":1323123057000},"page":"2733-2746","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":109,"title":["Immature B cells preferentially switch to IgE with increased direct S\u03bc to S\u03b5 recombination"],"prefix":"10.1084","volume":"208","author":[{"given":"Duane R.","family":"Wesemann","sequence":"first","affiliation":[{"name":"Program in Cellular and Molecular Medicine and Immune Disease Institute 1 , 2 , 3"},{"name":"Division of Immunology 1 , 2 , 3"},{"name":"Joint Program in Transfusion Medicine and the Department of Laboratory Medicine, Children\u2019s Hospital Boston, MA 02115 1 , 2 , 3"},{"name":"Department of Genetics, Harvard Medical School, Boston, MA 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