{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:28:54Z","timestamp":1791412134400,"version":"4.3.3"},"reference-count":45,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,5,6]]},"abstract":"<jats:p>Dendritic cells (DCs) represent a heterogeneous population of antigen-presenting cells that initiate and orient immune responses in secondary lymphoid organs. In mice, lymphoid organ\u2013resident CD8+ DCs are specialized at cross-presentation and have developed specific adaptations of their endocytic pathway (high pH, low degradation, and high export to the cytosol). In humans, blood BDCA3+ DCs were recently shown to be the homologues of mouse CD8+ DCs. They were also proposed to cross-present antigens more efficiently than other blood DC subsets after in vitro activation, suggesting that in humans cross-presentation is restricted to certain DC subsets. The DCs that cross-present antigen physiologically, however, are the ones present in lymphoid organs. Here, we show that freshly isolated tonsil-resident BDCA1+ DCs, BDCA3+ DCs, and pDCs all cross-present soluble antigen efficiently, as compared to macrophages, in the absence of activation. In addition, BDCA1+ and BDCA3+ DCs display similar phagosomal pH and similar production of reactive oxygen species in their phagosomes. All three DC subsets, in contrast to macrophages, also efficiently export internalized proteins to the cytosol. We conclude that all freshly isolated lymphoid organ\u2013resident human DCs, but not macrophages, display high intrinsic cross-presentation capacity.<\/jats:p>","DOI":"10.1084\/jem.20121103","type":"journal-article","created":{"date-parts":[[2013,4,8]],"date-time":"2013-04-08T21:50:21Z","timestamp":1365457821000},"page":"1035-1047","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":285,"title":["Similar antigen cross-presentation capacity and phagocytic functions in all freshly isolated human lymphoid organ\u2013resident dendritic cells"],"prefix":"10.1084","volume":"210","author":[{"given":"Elodie","family":"Segura","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U932, 75005 Paris, France 1"},{"name":"Institut Curie, Centre de Recherche, 75005 Paris, France 2"},{"name":"CBT507 IGR-Curie, INSERM Center of Clinical Investigation, 75005 Paris, France 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lanie","family":"Durand","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U932, 75005 Paris, France 1"},{"name":"Institut Curie, Centre de Recherche, 75005 Paris, France 2"},{"name":"CBT507 IGR-Curie, INSERM Center of Clinical Investigation, 75005 Paris, France 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Amigorena","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U932, 75005 Paris, France 1"},{"name":"Institut Curie, Centre de Recherche, 75005 Paris, France 2"},{"name":"CBT507 IGR-Curie, INSERM Center of Clinical Investigation, 75005 Paris, France 3"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2013,4,8]]},"reference":[{"key":"2023072601492258600_bib1","doi-asserted-by":"publisher","first-page":"1273","DOI":"10.1084\/jem.20100348","article-title":"Superior antigen cross-presentation and XCR1 expression define human CD11c+CD141+ cells as homologues of mouse CD8+ dendritic cells","volume":"207","author":"Bachem","year":"2010","journal-title":"J. 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