{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:33:52Z","timestamp":1791239632132,"version":"4.1.0"},"reference-count":32,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,6,2]]},"abstract":"<jats:p>Fanconi anemia is a rare genetic disorder that can lead to bone marrow failure, congenital abnormalities, and increased risk for leukemia and cancer. Cells with loss-of-function mutations in the FANC pathway are characterized by chromosome fragility, altered mutability, and abnormal regulation of the nonhomologous end-joining (NHEJ) pathway. Somatic hypermutation (SHM) and immunoglobulin (Ig) class switch recombination (CSR) enable B cells to produce high-affinity antibodies of various isotypes. Both processes are initiated after the generation of dG:dU mismatches by activation-induced cytidine deaminase. Whereas SHM involves an error-prone repair process that introduces novel point mutations into the Ig gene, the mismatches generated during CSR are processed to create double-stranded breaks (DSBs) in DNA, which are then repaired by the NHEJ pathway. As several lines of evidence suggest a possible role for the FANC pathway in SHM and CSR, we analyzed both processes in B cells derived from Fanca\u2212\/\u2212 mice. Here we show that Fanca is required for the induction of transition mutations at A\/T residues during SHM and that despite globally normal CSR function in splenic B cells, Fanca is required during CSR to stabilize duplexes between pairs of short microhomology regions, thereby impeding short-range recombination downstream of DSB formation.<\/jats:p>","DOI":"10.1084\/jem.20131637","type":"journal-article","created":{"date-parts":[[2014,5,6]],"date-time":"2014-05-06T05:38:37Z","timestamp":1399354717000},"page":"1011-1018","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":26,"title":["Fanca deficiency reduces A\/T transitions in somatic hypermutation and alters class switch recombination junctions in mouse B cells"],"prefix":"10.1084","volume":"211","author":[{"given":"Thuy Vy","family":"Nguyen","sequence":"first","affiliation":[{"name":"Centre National de la Recherche Scientifique UMR 8200, Institut Gustave Roussy, 94805 Villejuif, France 1"},{"name":"Universit\u00e9 Paris Sud, 91400 Orsay, France 2"},{"name":"Programme Equipe Labellis\u00e9es, Ligue Contre le Cancer, 75013 Paris, France 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia","family":"Riou","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Sud, 91400 Orsay, France 2"},{"name":"Laboratoire de Radiopathologie, Service Cellules Souches et Radiation, Institut de Radiobiologie Cellulaire et Mol\u00e9culaire, Direction des Sciences du Vivant, Commissariat \u00e0 L\u2019\u00e9nergie Atomique et aux \u00c9nergies Alternatives, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U967, 92265 Fontenay-aux-Roses, France 4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa\u00efd","family":"Aoufouchi","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique UMR 8200, Institut Gustave Roussy, 94805 Villejuif, France 1"},{"name":"Universit\u00e9 Paris Sud, 91400 Orsay, France 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Rosselli","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique UMR 8200, Institut Gustave Roussy, 94805 Villejuif, France 1"},{"name":"Universit\u00e9 Paris Sud, 91400 Orsay, France 2"},{"name":"Programme Equipe Labellis\u00e9es, Ligue Contre le Cancer, 75013 Paris, France 3"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2014,5,5]]},"reference":[{"key":"2023072602123678100_bib1","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.molcel.2010.06.026","article-title":"Preventing nonhomologous end joining suppresses DNA repair defects of Fanconi anemia","volume":"39","author":"Adamo","year":"2010","journal-title":"Mol. 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