{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T07:33:12Z","timestamp":1776929592161,"version":"3.51.2"},"reference-count":48,"publisher":"Rockefeller University Press","issue":"10","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,9,22]]},"abstract":"<jats:p>Myeloid cells are important contributors to arteriogenesis, but their key molecular triggers and cellular effectors are largely unknown. We report, in inflammatory monocytes, that the combination of chemokine receptor (CCR2) and adhesion receptor (\u03b22 integrin) engagement leads to an interaction between activated Rac2 and Myosin 9 (Myh9), the heavy chain of Myosin IIA, resulting in augmented vascular endothelial growth factor A (VEGF-A) expression and induction of arteriogenesis. In human monocytes, CCL2 stimulation coupled to ICAM-1 adhesion led to rapid nuclear-to-cytosolic translocation of the RNA-binding protein HuR. This activation of HuR and its stabilization of VEGF-A mRNA were Rac2-dependent, and proteomic analysis for Rac2 interactors identified the 226 kD protein Myh9. The level of induced Rac2\u2013Myh9 interaction strongly correlated with the degree of HuR translocation. CCL2-coupled ICAM-1 adhesion-driven HuR translocation and consequent VEGF-A mRNA stabilization were absent in Myh9\u2212\/\u2212 macrophages. Macrophage VEGF-A production, ischemic tissue VEGF-A levels, and flow recovery to hind limb ischemia were impaired in myeloid-specific Myh9\u2212\/\u2212 mice, despite preserved macrophage recruitment to the ischemic muscle. Micro-CT arteriography determined the impairment to be defective induced arteriogenesis, whereas developmental vasculogenesis was unaffected. These results place the macrophage at the center of ischemia-induced arteriogenesis, and they establish a novel role for Myosin IIA in signal transduction events modulating VEGF-A expression in tissue.<\/jats:p>","DOI":"10.1084\/jem.20132130","type":"journal-article","created":{"date-parts":[[2014,9,2]],"date-time":"2014-09-02T04:19:08Z","timestamp":1409631548000},"page":"1957-1968","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":50,"title":["Chemokine-coupled \u03b22 integrin\u2013induced macrophage Rac2\u2013Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis"],"prefix":"10.1084","volume":"211","author":[{"given":"Alan R.","family":"Morrison","sequence":"first","affiliation":[{"name":"Section of Cardiovascular Medicine, Department of Internal Medicine and the Yale Cardiovascular Research Center 1 , 2 , 3 , and 4"},{"name":"Department of Immunobiology 1 , 2 , 3 , and 4"},{"name":"Department of Cell 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