{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:24:11Z","timestamp":1791228251578,"version":"4.1.0"},"reference-count":49,"publisher":"Rockefeller University Press","issue":"10","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,9,22]]},"abstract":"<jats:p>Hematopoiesis is fully dependent on hematopoietic stem cells (HSCs) that possess the capacity to self-renew and differentiate into all blood cell lineages. WASH, Wiskott\u2013Aldrich syndrome protein (WASP) and SCAR homologue (WASH) is involved in endosomal sorting as an actin-nucleating protein. Here, we show that conditional WASH deletion in the hematopoietic system causes defective blood production of the host, leading to severe cytopenia and rapid anemia. WASH deficiency causes the accumulation of long-term (LT)-HSCs in bone marrow and perturbs their differentiation potential to mature blood lineages. Importantly, WASH is located in the nucleus of LT-HSCs and associates with the nucleosome remodeling factor (NURF) complex. WASH assists the NURF complex to the promoter of c-Myc gene through its VCA domain-dependent nuclear actin nucleation. WASH deletion suppresses the transcriptional activation of c-Myc gene and impairs the differentiation of LT-HSCs. WASH acts as an upstream regulator to modulate c-Myc transcription for hematopoietic regulation.<\/jats:p>","DOI":"10.1084\/jem.20140169","type":"journal-article","created":{"date-parts":[[2014,9,16]],"date-time":"2014-09-16T07:56:27Z","timestamp":1410854187000},"page":"2119-2134","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":65,"title":["WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc\u2013dependent manner"],"prefix":"10.1084","volume":"211","author":[{"given":"Pengyan","family":"Xia","sequence":"first","affiliation":[{"name":"Key Laboratory of Infection and Immunity of CAS, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 1"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Infection and Immunity of CAS, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanling","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Infection and Immunity of CAS, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 1"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingping","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Infection and Immunity of CAS, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Infection and Immunity of CAS, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 1"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Buqing","family":"Ye","sequence":"additional","affiliation":[{"name":"Key Laboratory of Infection and Immunity of CAS, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Du","sequence":"additional","affiliation":[{"name":"Key Laboratory of Infection and Immunity of CAS, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zusen","family":"Fan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Infection and Immunity of CAS, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2014,9,15]]},"reference":[{"key":"2023072601553226300_bib1","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1182\/blood-2002-06-1780","article-title":"Transcriptional accessibility for genes of multiple tissues and hematopoietic lineages is hierarchically controlled during early hematopoiesis","volume":"101","author":"Akashi","year":"2003","journal-title":"Blood."},{"key":"2023072601553226300_bib2","doi-asserted-by":"publisher","first-page":"3197","DOI":"10.1016\/j.febslet.2011.09.003","article-title":"The nucleosome remodeling factor","volume":"585","author":"Alkhatib","year":"2011","journal-title":"FEBS Lett."},{"key":"2023072601553226300_bib3","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1101\/gad.1787609","article-title":"An operational definition of epigenetics","volume":"23","author":"Berger","year":"2009","journal-title":"Genes Dev."},{"key":"2023072601553226300_bib4","doi-asserted-by":"publisher","first-page":"14541","DOI":"10.1073\/pnas.261562798","article-title":"Flk-2 is a marker in hematopoietic stem cell differentiation: a simple method to isolate long-term stem cells","volume":"98","author":"Christensen","year":"2001","journal-title":"Proc. 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