{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:12:26Z","timestamp":1791231146801,"version":"4.1.0"},"reference-count":79,"publisher":"Rockefeller University Press","issue":"8","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,7,27]]},"abstract":"<jats:p>Neutrophil recruitment, mediated by \u03b22 integrins, combats pyogenic infections but also plays a key role in ischemia\u2013reperfusion injury and other inflammatory disorders. Talin induces allosteric rearrangements in integrins that increase affinity for ligands (activation). Talin also links integrins to actin and other proteins that enable formation of adhesions. Structural studies have identified a talin1 mutant (L325R) that perturbs activation without impairing talin\u2019s capacity to link integrins to actin and other proteins. Here, we found that mice engineered to express only talin1(L325R) in myeloid cells were protected from renal ischemia\u2013reperfusion injury. Dissection of neutrophil function in vitro and in vivo revealed that talin1(L325R) neutrophils had markedly impaired chemokine-induced, \u03b22 integrin\u2013mediated arrest, spreading, and migration. Surprisingly, talin1(L325R) neutrophils exhibited normal selectin-induced, \u03b22 integrin\u2013mediated slow rolling, in sharp contrast to the defective slow rolling of neutrophils lacking talin1 or expressing a talin1 mutant (W359A) that blocks talin interaction with integrins. These studies reveal the importance of talin-mediated activation of integrins for renal ischemia\u2013reperfusion injury. They further show that neutrophil arrest requires talin recruitment to and activation of integrins. However, although neutrophil slow rolling requires talin recruitment to integrins, talin-mediated integrin activation is dispensable.<\/jats:p>","DOI":"10.1084\/jem.20142358","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T02:04:42Z","timestamp":1436839482000},"page":"1267-1281","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":87,"title":["Blocking neutrophil integrin activation prevents ischemia\u2013reperfusion injury"],"prefix":"10.1084","volume":"212","author":[{"given":"Tadayuki","family":"Yago","sequence":"first","affiliation":[{"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian G.","family":"Petrich","sequence":"additional","affiliation":[{"name":"Department of Pediatrics 3 and 4"},{"name":"Aflac Cancer and Blood Disorders Center, Emory University School of Medicine, Atlanta, GA 30322 3 and 4"},{"name":"Department of Pediatrics 3 and 4"},{"name":"Aflac Cancer and Blood Disorders Center, Emory University School of Medicine, Atlanta, GA 30322 3 and 4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenghui","family":"Liu","sequence":"additional","affiliation":[{"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bojing","family":"Shao","sequence":"additional","affiliation":[{"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark H.","family":"Ginsberg","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California at San Diego, La Jolla, CA 92093 5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodger P.","family":"McEver","sequence":"additional","affiliation":[{"name":"Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104 1"},{"name":"Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104 2"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2015,7,13]]},"reference":[{"key":"2023072603111385300_bib1","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1146\/annurev.immunol.021908.132554","article-title":"The ins and outs of leukocyte integrin signaling","volume":"27","author":"Abram","year":"2009","journal-title":"Annu. 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