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We have previously shown that macrophage colony-stimulating factor (CSF-1; M-CSF) directly instructed myeloid commitment in HSCs. In this study, we tested whether this effect had therapeutic benefit in improving protection against pathogens after HS\/PC transplantation. M-CSF treatment resulted in an increased production of mature myeloid donor cells and an increased survival of recipient mice infected with lethal doses of clinically relevant opportunistic pathogens, namely the bacteria Pseudomonas aeruginosa and the fungus Aspergillus fumigatus. M-CSF treatment during engraftment or after infection efficiently protected from these pathogens as early as 3 days after transplantation and was effective as a single dose. It was more efficient than granulocyte CSF (G-CSF), a common treatment of severe neutropenia, which showed no protective effect under the tested conditions. M-CSF treatment showed no adverse effect on long-term lineage contribution or stem cell activity and, unlike G-CSF, did not impede recovery of HS\/PCs, thrombocyte numbers, or glucose metabolism. These results encourage potential clinical applications of M-CSF to prevent severe infections after HS\/PC transplantation.<\/jats:p>","DOI":"10.1084\/jem.20151975","type":"journal-article","created":{"date-parts":[[2016,10,17]],"date-time":"2016-10-17T09:44:52Z","timestamp":1476697492000},"page":"2269-2279","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":46,"title":["M-CSF improves protection against bacterial and fungal infections after hematopoietic stem\/progenitor cell transplantation"],"prefix":"10.1084","volume":"213","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5405-7286","authenticated-orcid":false,"given":"Prashanth K.","family":"Kandalla","sequence":"first","affiliation":[{"name":"Centre National de la Recherche Scientifique, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Universit\u00e9, 13288 Marseille, France 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