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Whether IL-7 is a continued requirement for normal lymph node structure and function is unknown. To address this, we ablated IL-7 function in normal adult hosts. Either inducible Il7 gene deletion or IL-7R blockade in adults resulted in a rapid loss of lymph node cellularity and a corresponding defect in lymphocyte entry into lymph nodes. Although stromal and dendritic cell components of lymph nodes were present in normal numbers and representation, innate lymphoid cell (ILC) subpopulations were substantially decreased after IL-7 ablation. Testing lymphocyte homing in bone marrow chimeras reconstituted with Rorc\u2212\/\u2212 bone marrow confirmed that ILC3s in lymph nodes are required for normal lymphocyte homing. Collectively, our data suggest that maintenance of intact lymph nodes relies on IL-7\u2013dependent maintenance of ILC3 cells.<\/jats:p>","DOI":"10.1084\/jem.20170518","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T10:22:14Z","timestamp":1519381334000},"page":"1069-1077","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":44,"title":["IL-7\u2013dependent maintenance of ILC3s is required for normal entry of lymphocytes into lymph nodes"],"prefix":"10.1084","volume":"215","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9157-0532","authenticated-orcid":true,"given":"Jie","family":"Yang","sequence":"first","affiliation":[{"name":"Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital, London, England, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5440-1067","authenticated-orcid":true,"given":"Ferry","family":"Cornelissen","sequence":"additional","affiliation":[{"name":"Department of Hematology, Erasmus University Medical Center, Rotterdam, Netherlands 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8616-5652","authenticated-orcid":true,"given":"Natalie","family":"Papazian","sequence":"additional","affiliation":[{"name":"Department of Hematology, Erasmus University Medical Center, Rotterdam, Netherlands 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7471-7715","authenticated-orcid":true,"given":"Rogier M.","family":"Reijmers","sequence":"additional","affiliation":[{"name":"Department of Hematology, Erasmus University Medical Center, Rotterdam, Netherlands 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Llorian","sequence":"additional","affiliation":[{"name":"The Francis Crick Institute, Kings Cross, London, England, UK 4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1095-4273","authenticated-orcid":true,"given":"Tom","family":"Cupedo","sequence":"additional","affiliation":[{"name":"Department of Hematology, Erasmus University Medical Center, Rotterdam, Netherlands 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8079-9358","authenticated-orcid":true,"given":"Mark","family":"Coles","sequence":"additional","affiliation":[{"name":"Kennedy Institute of Rheumatology, University of Oxford, Oxford, England, UK 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4352-3373","authenticated-orcid":true,"given":"Benedict","family":"Seddon","sequence":"additional","affiliation":[{"name":"Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital, London, England, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2018,2,22]]},"reference":[{"key":"2023072604195088100_bib1","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1038\/nature13814","article-title":"Dendritic cells control fibroblastic reticular network tension and lymph node expansion","volume":"514","author":"Acton","year":"2014","journal-title":"Nature."},{"key":"2023072604195088100_bib2","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1084\/jem.20031975","article-title":"A role for thymic stromal lymphopoietin in CD4(+) T cell development","volume":"200","author":"Al-Shami","year":"2004","journal-title":"J. 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