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It has been postulated that repression of Pax5 by Blimp1 is essential for plasma cell development. Here, we challenged this hypothesis by analyzing the IghPax5\/+ mouse, which expressed a Pax5 minigene from the immunoglobulin heavy-chain locus. Despite high Pax5 expression, plasma cells efficiently developed in young IghPax5\/+ mice at steady state and upon immunization, while their number moderately declined in older mice. Although Pax5 significantly deregulated the plasma cell expression program, key plasma cell regulators were normally expressed in IghPax5\/+ plasma cells. While IgM and IgA secretion by IghPax5\/+ plasma cells was normal, IgG secretion was modestly decreased. Hence, Pax5 repression is not essential for robust plasma cell development and antibody secretion, although it is required for optimal IgG production and accumulation of long-lived plasma cells.<\/jats:p>","DOI":"10.1084\/jem.20200147","type":"journal-article","created":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T21:03:32Z","timestamp":1597179812000},"update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":30,"title":["Repression of the B cell identity factor Pax5 is not required for plasma cell development"],"prefix":"10.1084","volume":"217","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3391-1199","authenticated-orcid":true,"given":"Grace J.","family":"Liu","sequence":"first","affiliation":[{"name":"Research Institute of Molecular Pathology, Vienna BioCenter, Vienna, Austria 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9326-2047","authenticated-orcid":true,"given":"Markus","family":"Jaritz","sequence":"additional","affiliation":[{"name":"Research Institute of Molecular Pathology, Vienna BioCenter, Vienna, Austria 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0965-5938","authenticated-orcid":true,"given":"Miriam","family":"W\u00f6hner","sequence":"additional","affiliation":[{"name":"Research Institute of Molecular Pathology, Vienna BioCenter, Vienna, Austria 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0044-8396","authenticated-orcid":true,"given":"Benedikt","family":"Agerer","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0597-1976","authenticated-orcid":true,"given":"Andreas","family":"Bergthaler","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7723-9579","authenticated-orcid":true,"given":"Stephen G.","family":"Malin","sequence":"additional","affiliation":[{"name":"Research Institute of Molecular Pathology, Vienna BioCenter, Vienna, Austria 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9111-9351","authenticated-orcid":true,"given":"Meinrad","family":"Busslinger","sequence":"additional","affiliation":[{"name":"Research Institute of Molecular Pathology, Vienna BioCenter, Vienna, Austria 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2020,8,11]]},"reference":[{"key":"2023072803213032800_bib1","doi-asserted-by":"publisher","first-page":"1589","DOI":"10.1101\/gad.6.9.1589","article-title":"Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis","volume":"6","author":"Adams","year":"1992","journal-title":"Genes Dev"},{"key":"2023072803213032800_bib2","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/j.cell.2013.01.007","article-title":"Mechanisms of programmed DNA lesions and genomic instability in the immune system","volume":"152","author":"Alt","year":"2013","journal-title":"Cell"},{"key":"2023072803213032800_bib3","doi-asserted-by":"publisher","first-page":"3318","DOI":"10.1093\/bioinformatics\/bts607","article-title":"MULTOVL: fast multiple overlaps of genomic regions","volume":"28","author":"Asz\u00f3di","year":"2012","journal-title":"Bioinformatics"},{"key":"2023072803213032800_bib4","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1046\/j.1440-1711.1998.00709.x","article-title":"Defective TCR expression in transgenic mice constructed using cDNA-based \u03b1- and \u03b2-chain genes under the control of heterologous regulatory elements","volume":"76","author":"Barnden","year":"1998","journal-title":"Immunol. 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