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Their contribution toward CeD pathogenesis is unclear. We assessed expression of markers associated with PC longevity in 15 untreated and 26 treated CeD patients in addition to 13 non-CeD controls and performed RNA sequencing with clonal inference and transcriptomic analysis of 3,251 single PCs. We observed antigen-dependent V-gene selection and stereotypic antibodies. Generation of recombinant DGP-specific antibodies revealed a key role of a heavy chain residue that displays polymorphism, suggesting that immunoglobulin gene polymorphisms may influence CeD-specific antibody responses. We identified transcriptional differences between CeD-specific and non\u2013disease-specific PCs and between short-lived and long-lived PCs. The short-lived CD19+CD45+ phenotype dominated in untreated and short-term\u2013treated CeD, in particular among disease-specific PCs but also in the general PC population. Thus, the disease lesion of untreated CeD is characterized by massive accumulation of short-lived PCs that are not only directed against disease-specific antigens.<\/jats:p>","DOI":"10.1084\/jem.20200852","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T02:22:12Z","timestamp":1601518932000},"update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":49,"title":["Longevity, clonal relationship, and transcriptional program of celiac disease\u2013specific plasma cells"],"prefix":"10.1084","volume":"218","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8343-2842","authenticated-orcid":true,"given":"Ida","family":"Lindeman","sequence":"first","affiliation":[{"name":"KG Jebsen Coeliac Disease Research Centre, University of Oslo, Oslo, Norway 1"},{"name":"Department of Immunology, University of Oslo and Oslo University Hospital, Oslo, Norway 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