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One hypothetical possibility is that limited CD4 T cell help to B cells in a normal germinal center (GC) response results in selective recruitment of abundant, immunodominant B cells. This is a central issue in HIV envelope glycoprotein (Env) vaccine designs, because precursors to broadly neutralizing epitopes are rare. Here, we sought to elucidate whether modulating the quantity of T cell help can influence recruitment and competition of broadly neutralizing antibody precursor B cells at a physiological precursor frequency in response to Env trimer immunization. To do so, two new Env-specific CD4 transgenic (Tg) T cell receptor (TCR) mouse lines were generated, carrying TCR pairs derived from Env-protein immunization. Our results suggest that CD4 T cell help quantitatively regulates early recruitment of rare B cells to GCs.<\/jats:p>","DOI":"10.1084\/jem.20201254","type":"journal-article","created":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T15:23:14Z","timestamp":1608736994000},"update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":61,"title":["Modulating the quantity of HIV Env-specific CD4 T cell help promotes rare B cell responses in germinal centers"],"prefix":"10.1084","volume":"218","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7371-845X","authenticated-orcid":true,"given":"Jeong Hyun","family":"Lee","sequence":"first","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, CA 1"},{"name":"Consortium for HIV\/AIDS Vaccine Development, The Scripps Research Institute, La Jolla, CA 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