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Currently, the heterogeneous human cutaneous DC population is incompletely characterized, and its contribution to these diseases remains unclear. Here, we performed index-sorted single-cell flow cytometry and RNA sequencing of lesional and nonlesional AD and PSO skin to identify macrophages and all DC subsets, including the newly described mature LAMP3+BIRC3+ DCs enriched in immunoregulatory molecules (mregDC) and CD14+ DC3. By integrating our indexed data with published skin datasets, we generated a myeloid cell universe of DC and macrophage subsets in healthy and diseased skin. Importantly, we found that CD14+ DC3s increased in PSO lesional skin and co-produced IL1B and IL23A, which are pathological in PSO. Our study comprehensively describes the molecular characteristics of macrophages and DC subsets in AD and PSO at single-cell resolution, and identifies CD14+ DC3s as potential promoters of inflammation in PSO.<\/jats:p>","DOI":"10.1084\/jem.20202345","type":"journal-article","created":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T02:21:38Z","timestamp":1623810098000},"update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":174,"title":["Single-cell analysis of human skin identifies CD14+ type 3 dendritic cells co-producing IL1B and IL23A in psoriasis"],"prefix":"10.1084","volume":"218","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9332-0369","authenticated-orcid":true,"given":"Satoshi","family":"Nakamizo","sequence":"first","affiliation":[{"name":"Singapore Immunology Network, Agency for Science, Technology and Research, Singapore 1"},{"name":"Skin Research Institute of Singapore, Agency for Science, Technology and 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