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Administration of succinate production\u2013deficient microbes shows a reduced response in a Pou2f3-dependent manner despite similar intestinal colonization. Finally, antibiotic-treated mice prophylactically administered with succinate-producing bacteria show increased protection against C. difficile\u2013induced morbidity and mortality. This effect is nullified in Pou2f3\u2212\/\u2212 mice, confirming that the protection occurs via the TC pathway. We propose that activation of TCs by the microbiota in the colon is a mechanism evolved by the host to counterbalance microbiome-derived cues that facilitate invasion by pathogens.<\/jats:p>","DOI":"10.1084\/jem.20232055","type":"journal-article","created":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T02:20:51Z","timestamp":1729736451000},"update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":18,"title":["Succinate-producing microbiota drives tuft cell hyperplasia to protect against\n                    <i>Clostridioides difficile<\/i>"],"prefix":"10.1084","volume":"222","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6294-0098","authenticated-orcid":true,"given":"Tasia D.","family":"Kellogg","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0464eyp60","id-type":"ROR","asserted-by":"publisher"}],"name":"UMass Chan Medical School 1 Department of Microbiology, , Worcester, 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