{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T21:01:58Z","timestamp":1648674118556},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1931,11,1]]},"abstract":"<jats:p>1. When suspended in slightly alkaline (pH 7.4 to 7.8) saline dilutions of clear, hemoglobin-free normal monkey serum, the virus of yellow fever from infected monkeys and from infected, but blood-free, mosquitoes, usually acts as if it were possessed of a positive electrical charge.<\/jats:p>\n               <jats:p>2. The virus tends to assume a negative charge in fluids having a slightly acid reaction.<\/jats:p>\n               <jats:p>3. The isoelectric point of the virus seems to be in the neighborhood of pH 7.0, possibly ranging from pH 7.3 to pH 6.9.<\/jats:p>\n               <jats:p>4. Exposure to fluid having a reaction of pH 5.0 for 3 hours appeared to inactivate the virus.<\/jats:p>\n               <jats:p>5. In experiments in which the suspending fluid was prepared with normal serum diluted with distilled water and containing a good quantity of partly hemolyzed erythrocytes, the virus tended to migrate to the anode.<\/jats:p>\n               <jats:p>6. The protective bodies in yellow fever immune serum appear to carry a negative charge in slightly alkaline saline dilutions of serum.<\/jats:p>","DOI":"10.1084\/jem.54.5.733","type":"journal-article","created":{"date-parts":[[2004,6,18]],"date-time":"2004-06-18T06:19:10Z","timestamp":1087539550000},"page":"733-745","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":2,"title":["ELECTROPHORESIS EXPERIMENTS WITH THE VIRUS AND PROTECTIVE BODIES OF YELLOW FEVER"],"prefix":"10.1084","volume":"54","author":[{"suffix":"Jr.","given":"Martin","family":"Frobisher","sequence":"first","affiliation":[{"name":"From the Yellow Fever Laboratory of the International Health Division of the Rockefeller Foundation, Bahia, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1931,11,1]]},"container-title":["Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/rupress.org\/jem\/article-pdf\/54\/5\/733\/1179395\/733.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/rupress.org\/jem\/article-pdf\/54\/5\/733\/1179395\/733.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T12:37:35Z","timestamp":1601728655000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/54\/5\/733\/10224\/ELECTROPHORESIS-EXPERIMENTS-WITH-THE-VIRUS-AND"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1931,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1931,11,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.54.5.733","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[1931,11,1]]}}}