{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:19:29Z","timestamp":1791425969981,"version":"4.3.3"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1937,6,1]]},"abstract":"<jats:p>1. In a search for suitable tissues for the cultivation of yellow fever virus in vitro, mouse embryos were inoculated with this virus in utero. A titration for virus content of the various organs of the embryos indicated that the virus was present in the brain in greatest concentration.<\/jats:p>\n               <jats:p>2. Unmodified strains of yellow fever virus were readily adapted to cultivation in vitro in a medium consisting of minced mouse embryo brain tissue and Tyrode solution containing 10 per cent normal monkey serum.<\/jats:p>\n               <jats:p>3. After a continued cultivation in mouse embryo brain tissue cultures for twenty to twenty-five subcultures, these strains were readily adapted to cultivation in whole mouse embryo tissue medium.<\/jats:p>\n               <jats:p>4. There is evidence to indicate that a prolonged cultivation of the virus in mouse embryo brain medium increases its neurotropic properties.<\/jats:p>\n               <jats:p>5. Attempts to employ monkey tissues for in vitro cultivation of yellow fever virus gave entirely negative results.<\/jats:p>","DOI":"10.1084\/jem.65.6.801","type":"journal-article","created":{"date-parts":[[2004,6,21]],"date-time":"2004-06-21T23:43:35Z","timestamp":1087861415000},"page":"801-808","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":26,"title":["THE ADAPTATION OF UNMODIFIED STRAINS OF YELLOW FEVER VIRUS TO CULTIVATION IN VITRO"],"prefix":"10.1084","volume":"65","author":[{"given":"Hugh H.","family":"Smith","sequence":"first","affiliation":[{"name":"From the Laboratories of the International Health Division, The Rockefeller Foundation, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Theiler","sequence":"additional","affiliation":[{"name":"From the Laboratories of the International Health Division, The Rockefeller Foundation, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1937,6,1]]},"container-title":["Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/rupress.org\/jem\/article-pdf\/65\/6\/801\/1180981\/801.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/rupress.org\/jem\/article-pdf\/65\/6\/801\/1180981\/801.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T13:06:02Z","timestamp":1601730362000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/65\/6\/801\/26192\/THE-ADAPTATION-OF-UNMODIFIED-STRAINS-OF-YELLOW"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1937,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1937,6,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.65.6.801","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[1937,6,1]]}}}