{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T14:13:50Z","timestamp":1756995230341},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1941,2,1]]},"abstract":"<jats:p>1. Quantitative precipitin studies indicate that progressive photo-oxidation progressively destroys the antigenic function of egg albumin.<\/jats:p>\n               <jats:p>2. Quantitative precipitin reactions of antisera (anti-egg albumin rabbit serum and antipneumococcus Type I horse serum) demonstrate that progressive photo-oxidation causes progressive lowering of the potency of the sera.<\/jats:p>\n               <jats:p>3. Quantitative precipitin reactions of the photo-oxidized globulin gamma fraction of anti-egg albumin rabbit serum and of Felton solution of antipneumococcus Type I horse serum show that these specific antibody fractions behave similarly to antibodies in whole sera.<\/jats:p>\n               <jats:p>4. Egg albumin whose precipitin reaction is destroyed by photo-oxidation no longer causes anaphylaxis in guinea pigs and does not produce precipitins in rabbits.<\/jats:p>\n               <jats:p>5. Chemical studies of progressively photo-oxidized egg albumin show a progressive destruction of tryptophane and histidine while tyrosine remains intact and cystine is reversibly oxidized. Sulfhydryl groups can no longer be demonstrated in photo-oxidized egg albumin whose antigenic characteristics are greatly weakened.<\/jats:p>\n               <jats:p>6. Similar studies on the globulin gamma fraction of anti-egg albumin rabbit serum and on Felton solution show no diminution of these amino acids in photo-oxidized material whose antigenic properties are destroyed.<\/jats:p>\n               <jats:p>7. The non-coagulable nitrogen and the amino nitrogen of egg albumin, antisera, and their specific antibody fractions show but an insignificant increase during photo-oxidation, indicating that the loss of the precipitin reaction is not due to splitting of the respective protein molecules.<\/jats:p>\n               <jats:p>8. Electrophoretic studies of egg albumin, antisera, and their specific antibody fractions show that photo-oxidation causes a marked alteration of the pattern of these substrates.<\/jats:p>\n               <jats:p>9. Photo-oxidation of proteins causes the formation of aggregates, indicating denaturation.<\/jats:p>\n               <jats:p>10. Hematoporphyrin migrates with the albumin fraction of unaltered as well as the photo-oxidized anti-egg albumin rabbit serum and pneumococcus Type I horse serum; in isolated proteins such as egg albumin, globulin gamma, or Felton solution, etc., the dye moves independently of the protein; after progressive photo-oxidation Hp becomes progressively fixed to the protein. Eosin behaves similarly to hematoporphyrin.<\/jats:p>","DOI":"10.1084\/jem.73.2.223","type":"journal-article","created":{"date-parts":[[2004,6,21]],"date-time":"2004-06-21T23:43:35Z","timestamp":1087861415000},"page":"223-242","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":20,"title":["STUDIES ON PHOTO-OXIDATION OF ANTIGEN AND ANTIBODIES"],"prefix":"10.1084","volume":"73","author":[{"given":"Hans","family":"Smetana","sequence":"first","affiliation":[{"name":"From the Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, and the Physico-Chemical Institute, University of Upsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Shemin","sequence":"additional","affiliation":[{"name":"From the Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, and the Physico-Chemical Institute, University of Upsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1941,2,1]]},"container-title":["Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/rupress.org\/jem\/article-pdf\/73\/2\/223\/1182094\/223.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/rupress.org\/jem\/article-pdf\/73\/2\/223\/1182094\/223.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T13:21:15Z","timestamp":1601731275000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/73\/2\/223\/4487\/STUDIES-ON-PHOTOOXIDATION-OF-ANTIGEN-AND"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1941,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1941,2,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.73.2.223","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[1941,2,1]]}}}