{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T09:06:35Z","timestamp":1761987995790},"reference-count":0,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1964,10,1]]},"abstract":"<jats:p>The number of total disulfide bonds in rabbit and sheep 7S gamma globulin, before and after treatment with 2-mercaptoethanol, has been measured by amperometric titration.<\/jats:p>\n               <jats:p>Mercaptan reduction could diminish the complement-fixing efficiency of 7S rabbit gamma globulin by no more than 90 per cent without any significant decrease in maximal complement-fixing ability. This was associated with the reduction of only 7 disulfide bonds, including 1 interchain disulfide bond [S\u2014S(1)].<\/jats:p>\n               <jats:p>The reduction of 7 disulfide bonds in 7S sheep gamma globulin was associated with a 64 per cent decrease in complement-fixing efficiency, while reduction of 10 to 11 disulfide bonds decreased the complement-fixing efficiency by 90 per cent. Reduction of more disulfide bonds was not associated with any further decrease in complement-fixing efficiency, nor of any decrease in maximal complement-fixing ability.<\/jats:p>\n               <jats:p>The reduction of more than 10 to 11 disulfide bonds in 7S rabbit and sheep gamma globulins was associated with a decrease in precipitating ability.<\/jats:p>\n               <jats:p>The disulfide bonds which are more labile to mercaptan reduction appear to be associated with complement-fixing efficiency while the disulfide bonds which are more resistant to mercaptan reduction appear to be associated with precipitating ability.<\/jats:p>\n               <jats:p>The measurement of interpolypeptide chain disulfide bonds has been discussed. One easily reduced interchain disulfide bond [S\u2014S(1)] appears to be important for the complement-fixing efficiency of 7S rabbit antibody. The integrity of this same bond is essential for the precipitating ability of 5S rabbit antibody and may also be important for its complement-fixing ability.<\/jats:p>","DOI":"10.1084\/jem.120.4.531","type":"journal-article","created":{"date-parts":[[2004,6,22]],"date-time":"2004-06-22T20:51:07Z","timestamp":1087937467000},"page":"531-545","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":61,"title":["THE ROLE OF DISULFIDE BONDS IN THE COMPLEMENT-FIXING AND PRECIPITATING PROPERTIES OF 7S RABBIT AND SHEEP ANTIBODIES"],"prefix":"10.1084","volume":"120","author":[{"given":"Peter H.","family":"Schur","sequence":"first","affiliation":[{"name":"From the Department of Immunochemistry and Division of Biochemistry, Walter Reed Army Institute of Research, Washington, D. C."}]},{"given":"Gary D.","family":"Christian","sequence":"additional","affiliation":[{"name":"From the Department of Immunochemistry and Division of Biochemistry, Walter Reed Army Institute of Research, Washington, D. C."}]}],"member":"291","published-online":{"date-parts":[[1964,10,1]]},"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/120\/4\/531\/1650324\/531.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/120\/4\/531\/1650324\/531.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T19:39:35Z","timestamp":1690227575000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/120\/4\/531\/3771\/THE-ROLE-OF-DISULFIDE-BONDS-IN-THE-COMPLEMENT"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1964,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1964,10,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.120.4.531","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"value":"1540-9538","type":"electronic"},{"value":"0022-1007","type":"print"}],"subject":[],"published":{"date-parts":[[1964,10,1]]}}}