{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T18:13:15Z","timestamp":1761934395943,"version":"build-2065373602"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1973,7,1]]},"abstract":"<jats:p>Spleen cells from CBA or congenitally athymic (\"nude\") mice were pretreated with various concentrations of DNP coupled to a copolymer of D-glutamic acid and D-lysine (DNP37-D-GL), under various conditions of time and temperature. After washing, they were then cultured for 3 days with the direct B cell immunogen, DNP coupled to Salmonella adelaide flagella (DNP-FLA). Under all circumstances tried, exposure of cells to 1 \u00b5g\/ml DNP-D-GL caused a 70\u2013100% depression in the subsequent DNP-specific PFC response, and 100 ng\/ml caused a lesser but still substantial effect. At the concentrations used, DNP-D-GL did not affect irrelevant antibody responses. Though cells from nude mice responded somewhat less well to DNP-FLA than those from CBA mice, no significant difference in the reaction of the two populations to the tolerogen was noted. This demonstrates that DNP-D-GL can, as previously suspected, directly cause unresponsiveness in B lymphocytes.<\/jats:p>","DOI":"10.1084\/jem.138.1.312","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T23:23:31Z","timestamp":1088033011000},"page":"312-317","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":44,"title":["INDUCTION OF B CELL TOLERANCE IN VITRO TO 2,4-DINITROPHENYL COUPLED TO A COPOLYMER OF <scp>D<\/scp>-GLUTAMIC ACID AND <scp>D<\/scp>-LYSINE (DNP-<scp>D<\/scp>-GL)"],"prefix":"10.1084","volume":"138","author":[{"given":"G. J. V.","family":"Nossal","sequence":"first","affiliation":[{"name":"From The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia and the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beverley L.","family":"Pike","sequence":"additional","affiliation":[{"name":"From The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia and the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David H.","family":"Katz","sequence":"additional","affiliation":[{"name":"From The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia and the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1973,7,1]]},"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/138\/1\/312\/1655585\/312.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/138\/1\/312\/1655585\/312.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T21:08:17Z","timestamp":1690232897000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/138\/1\/312\/6426\/INDUCTION-OF-B-CELL-TOLERANCE-IN-VITRO-TO-2-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1973,7,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.138.1.312","relation":{},"ISSN":["1540-9538","0022-1007"],"issn-type":[{"type":"electronic","value":"1540-9538"},{"type":"print","value":"0022-1007"}],"subject":[],"published":{"date-parts":[[1973,7,1]]}}}