{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:46:49Z","timestamp":1761058009316},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1975,5,1]]},"abstract":"<jats:p>The rat basophilic leukemia cell line (RBL-1) showed an inverse relationship between growth rate and expression of receptor activity for IgE. After prolonged exponential growth, the number of receptors per cell stabilized at 4-6 times 10-5. Cells in stationary cultures, which are arrested in the G1 phase of the cell cycle, continued to accumulate up to 0.9-1.7 times 10-6 receptors\/cell with no increase in volume. Upon resuspension in fresh medium at low density, these cells were shown to lose up to 70% of the receptor activity within 4 h. Assessment of cultures synchronized by double thymidine block and cells fractionated by centrifugation of a Ficoll gradient indicated that the RBL-1 cells acquire receptors in the G1 phase of the cell cycle. No accumulation of active receptors occurred during the S and G2 phases, though the average cell volume increased. Cell division resulted in a drop in number of receptors per cell while the number of cell-bound receptors in the culture remained unchanged. This indicates that during mitosis receptors were simply distributed to daughter cells.<\/jats:p>","DOI":"10.1084\/jem.141.5.1147","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T23:23:31Z","timestamp":1088033011000},"page":"1147-1162","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":46,"title":["Cell cycle-associated changes in receptors for IgE during growth and differentiation of a rat basophilic leukemia cell line."],"prefix":"10.1084","volume":"141","author":[{"given":"C","family":"Isersky","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Metzger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D N","family":"Buell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1975,5,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/141\/5\/1147\/1524435\/1147.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/141\/5\/1147\/1524435\/1147.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,22]],"date-time":"2023-07-22T14:27:16Z","timestamp":1690036036000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/141\/5\/1147\/21932\/Cell-cycle-associated-changes-in-receptors-for-IgE"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1975,5,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.141.5.1147","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,5,1]]}}}