{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T11:25:55Z","timestamp":1781177155403,"version":"3.54.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,9,1]]},"abstract":"<jats:p>Functional interactions between T and B lymphocytes are necessary for optimal activation of an immune response. Recently, the T lymphocyte receptor CD28 was shown to bind the B7 counter-receptor on activated B lymphocytes, and subsequently to costimulate interleukin 2 production and T cell proliferation. CTLA-4 is a predicted membrane receptor from cytotoxic T cells that is homologous to CD28 and whose gene maps to the same chromosomal band as the gene for CD28. It is not known, however, if CD28 and CTLA-4 also share functional properties. To investigate functional properties of CTLA-4, we have produced a soluble genetic fusion between the extracellular domain of CTLA-4 and an immunoglobulin C gamma chain. Here, we show that the fusion protein encoded by this construct, CTLA4Ig, bound specifically to B7-transfected Chinese hamster ovary cells and to lymphoblastoid cells. CTLA4Ig also immunoprecipitated B7 from cell surface 125I-labeled extracts of these cells. The avidity of 125I-labeled B7Ig fusion protein for immobilized CTLA4Ig was estimated (Kd approximately 12 nM). Finally, we show that CTLA4Ig was a potent inhibitor of in vitro immune responses dependent upon cellular interactions between T and B lymphocytes. These findings provide direct evidence that, like its structural homologue CD28, CTLA-4 is able to bind the B7 counter-receptor on activated B cells. Lymphocyte interactions involving the B7 counter-receptor are functionally important for alloantigen responses in vitro.<\/jats:p>","DOI":"10.1084\/jem.174.3.561","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"561-569","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":1287,"title":["CTLA-4 is a second receptor for the B cell activation antigen B7."],"prefix":"10.1084","volume":"174","author":[{"given":"P S","family":"Linsley","sequence":"first","affiliation":[{"name":"Oncogen Division, Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W","family":"Brady","sequence":"additional","affiliation":[{"name":"Oncogen Division, Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Urnes","sequence":"additional","affiliation":[{"name":"Oncogen Division, Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L S","family":"Grosmaire","sequence":"additional","affiliation":[{"name":"Oncogen Division, Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N K","family":"Damle","sequence":"additional","affiliation":[{"name":"Oncogen Division, Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J A","family":"Ledbetter","sequence":"additional","affiliation":[{"name":"Oncogen Division, Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1991,9,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/174\/3\/561\/1672335\/561.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/174\/3\/561\/1672335\/561.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:58:33Z","timestamp":1690250313000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/174\/3\/561\/24411\/CTLA-4-is-a-second-receptor-for-the-B-cell"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991,9,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.174.3.561","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,9,1]]}}}