{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:46:36Z","timestamp":1761057996692},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,12,1]]},"abstract":"<jats:p>We have previously described a set of mutants (16.23-selected mutants) of a B lymphoblastoid cell line that are defective in the presentation of intact proteins to class II-restricted T cells, but effectively present immunogenic peptides. The mutations in these mutants are recessive in somatic cell hybrids and are not in Class II structural genes. Here, we report on a unique mutant, 5.2.4, in which a similar defect in class II-restricted antigen presentation has occurred in association with a one-megabase homozygous deletion in the class II region of the major histocompatibility complex (MHC). The defects in class II presentation among three of the 16.23-selected mutants, and between these mutants and 5.2.4, are noncomplementary in somatic cell hybrids. This suggests that the class II presentation-defective phenotype in all four mutants results from lesions in a single MHC-linked gene, a conclusion strengthened by the finding that in a hybrid made with a second, unrelated MHC deletion mutant, T2, the class II presentation defect in a 16.23-selected mutant is also not complemented. Mutant 5.2.4, in addition to its class II presentation defect, is also defective in surface expression of MHC class I molecules, most likely because its deletion encompasses the peptide supply factor 1 gene, whose function is known to be required for normal abundance of cell surface class I molecules. However, the surface abundance of class I molecules is normal in the 16.23-selected mutants, suggesting that the lesions affecting class I surface abundance and class II presentation result from mutations in different genes.<\/jats:p>","DOI":"10.1084\/jem.174.6.1607","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1607-1615","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":79,"title":["A gene required for class II-restricted antigen presentation maps to the major histocompatibility complex."],"prefix":"10.1084","volume":"174","author":[{"given":"E","family":"Mellins","sequence":"first","affiliation":[{"name":"Department of Pediatrics, University of Washington 98105."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Kempin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Washington 98105."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Washington 98105."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Monji","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Washington 98105."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Pious","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Washington 98105."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1991,12,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/174\/6\/1607\/1672603\/1607.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/174\/6\/1607\/1672603\/1607.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T02:00:46Z","timestamp":1690250446000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/174\/6\/1607\/58167\/A-gene-required-for-class-II-restricted-antigen"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1991,12,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.174.6.1607","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,12,1]]}}}