{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:49:28Z","timestamp":1770720568470,"version":"3.49.0"},"reference-count":45,"publisher":"Rockefeller University Press","issue":"8","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,10,20]]},"abstract":"<jats:p>Previous studies have shown that integrin \u03b1 chain tails make strong positive contributions to integrin-mediated cell adhesion. We now show here that integrin \u03b14 tail deletion markedly impairs static cell adhesion by a mechanism that does not involve altered binding of soluble vascular cell adhesion molecule 1 ligand. Instead, truncation of the \u03b14 cytoplasmic domain caused a severe deficiency in integrin accumulation into cell surface clusters, as induced by ligand and\/ or antibodies. Furthermore, \u03b14 tail deletion also significantly decreased the membrane diffusivity of \u03b14\u03b21, as determined by a single particle tracking technique. Notably, low doses of cytochalasin D partially restored the deficiency in cell adhesion seen upon \u03b14 tail deletion. Together, these results suggest that \u03b14 tail deletion exposes the \u03b21 cytoplasmic domain, leading to cytoskeletal associations that apparently restrict integrin lateral diffusion and accumulation into clusters, thus causing reduced static cell adhesion. Our demonstration of integrin adhesive activity regulated through receptor diffusion\/clustering (rather than through altered ligand binding affinity) may be highly relevant towards the understanding of inside\u2013out signaling mechanisms for \u03b21 integrins.<\/jats:p>","DOI":"10.1084\/jem.186.8.1347","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"1347-1355","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":133,"title":["Mutational Evidence for Control of Cell Adhesion Through Integrin Diffusion\/Clustering, Independent of Ligand Binding"],"prefix":"10.1084","volume":"186","author":[{"given":"Robert L.","family":"Yauch","sequence":"first","affiliation":[{"name":"From the *Division of Tumor Virology, and the \u2021Division of Molecular and Cellular Biology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115; and the \u00a7Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan P.","family":"Felsenfeld","sequence":"additional","affiliation":[{"name":"From the *Division of Tumor Virology, and the \u2021Division of Molecular and Cellular Biology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115; and the \u00a7Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stine-Kathrein","family":"Kraeft","sequence":"additional","affiliation":[{"name":"From the *Division of Tumor Virology, and the \u2021Division of Molecular and Cellular Biology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115; and the \u00a7Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan Bo","family":"Chen","sequence":"additional","affiliation":[{"name":"From the *Division of Tumor Virology, and the \u2021Division of Molecular and Cellular Biology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115; and the \u00a7Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael P.","family":"Sheetz","sequence":"additional","affiliation":[{"name":"From the *Division of Tumor Virology, and the \u2021Division of Molecular and Cellular Biology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115; and the \u00a7Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin E.","family":"Hemler","sequence":"additional","affiliation":[{"name":"From the *Division of Tumor Virology, and the \u2021Division of Molecular and Cellular Biology, Dana-Farber Cancer Institute, 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