{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T08:41:22Z","timestamp":1766133682954},"reference-count":43,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,3,15]]},"abstract":"<jats:p>RNA splicing is an increasingly recognized regulator of immunity. Here, we demonstrate that after Mycobacterium tuberculosis infection (mRNA) il12rb1 is spliced by dendritic cells (DCs) to form an alternative (mRNA) il12rb1\u0394tm that encodes the protein IL-12R\u03b21\u0394TM. Compared with IL-12R\u03b21, IL-12R\u03b21\u0394TM contains an altered C-terminal sequence and lacks a transmembrane domain. Expression of IL-12R\u03b21\u0394TM occurs in CD11c+ cells in the lungs during M. tuberculosis infection. Selective reconstitution of il12rb1\u2212\/\u2212 DCs with (mRNA) il12rb1 and\/or (mRNA) il12rb1\u0394tm demonstrates that IL-12R\u03b21\u0394TM augments IL-12R\u03b21-dependent DC migration and activation of M. tuberculosis-specific T cells. It cannot mediate these activities independently of IL12R\u03b21. We hypothesize that M. tuberculosis-exposed DCs express IL-12R\u03b21\u0394TM to enhance IL-12R\u03b21-dependent migration and promote M. tuberculosis\u2013specific T cell activation. IL-12R\u03b21\u0394TM thus represents a novel positive-regulator of IL12R\u03b21-dependent DC function and of the immune response to M. tuberculosis.<\/jats:p>","DOI":"10.1084\/jem.20091085","type":"journal-article","created":{"date-parts":[[2010,3,9]],"date-time":"2010-03-09T05:09:31Z","timestamp":1268111371000},"page":"591-605","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":40,"title":["<i>Mycobacterium tuberculosis<\/i> infection induces <i>il12rb1<\/i> splicing to generate a novel IL-12R\u03b21 isoform that enhances DC migration"],"prefix":"10.1084","volume":"207","author":[{"given":"Richard T.","family":"Robinson","sequence":"first","affiliation":[{"name":"Trudeau Institute, Inc., Saranac Lake NY 12983 1"}]},{"given":"Shabaana A.","family":"Khader","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260 2"}]},{"given":"Cynthia A.","family":"Martino","sequence":"additional","affiliation":[{"name":"Trudeau Institute, Inc., Saranac Lake NY 12983 1"}]},{"given":"Jeffrey J.","family":"Fountain","sequence":"additional","affiliation":[{"name":"Trudeau Institute, Inc., Saranac Lake NY 12983 1"}]},{"given":"Maria","family":"Teixeira-Coelho","sequence":"additional","affiliation":[{"name":"Trudeau Institute, Inc., Saranac Lake NY 12983 1"},{"name":"Life and Health Sciences Research Institute, School of Health Sciences, University of Minho, 4710-057 Braga, Portugal 3"}]},{"given":"John E.","family":"Pearl","sequence":"additional","affiliation":[{"name":"Trudeau Institute, Inc., Saranac Lake NY 12983 1"}]},{"given":"Stephen T.","family":"Smiley","sequence":"additional","affiliation":[{"name":"Trudeau Institute, Inc., Saranac Lake NY 12983 1"}]},{"given":"Gary M.","family":"Winslow","sequence":"additional","affiliation":[{"name":"Trudeau Institute, Inc., Saranac Lake NY 12983 1"},{"name":"Wadsworth Center, New York State Department of Health, Albany, NY 12201 4"}]},{"given":"David L.","family":"Woodland","sequence":"additional","affiliation":[{"name":"Trudeau Institute, Inc., Saranac Lake NY 12983 1"}]},{"given":"Michael J.","family":"Walter","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, Washington University School of Medicine, St Louis, MO 63110 5"}]},{"given":"Jose R.","family":"Conejo-Garcia","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, NH 03756 6"}]},{"given":"Ueli","family":"Gubler","sequence":"additional","affiliation":[{"name":"Roche Discovery Technologies, Hoffmann-La Roche, Inc., Nutley, NJ 07110 7"}]},{"given":"Andrea M.","family":"Cooper","sequence":"additional","affiliation":[{"name":"Trudeau Institute, Inc., Saranac Lake NY 12983 1"}]}],"member":"291","published-online":{"date-parts":[[2010,3,8]]},"reference":[{"key":"2023072917485137900_bib1","doi-asserted-by":"publisher","first-page":"1432","DOI":"10.1126\/science.280.5368.1432","article-title":"Impairment of mycobacterial immunity in human interleukin-12 receptor deficiency","volume":"280","author":"Altare","year":"1998","journal-title":"Science."},{"key":"2023072917485137900_bib2","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1007\/s00439-007-0397-0","article-title":"Sequence variants in the genes for the interleukin-23 receptor (IL23R) and its ligand (IL12B) confer protection against psoriasis","volume":"122","author":"Capon","year":"2007","journal-title":"Hum. 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