{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:11:16Z","timestamp":1790691076800,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1993,7,1]]},"abstract":"<jats:p>The activity of apical membrane Na channels in the rat cortical collecting tubule was studied during manipulation of the animals' mineralocorticoid status in vivo using a low-Na diet or the diuretic furosemide. Tubules were isolated and split open to expose the luminal membrane surface. Induction of Na channel activity was studied in cell-attached patches of the split tubules. No activity was observed with control animals on a normal diet. Channel activity could be induced by putting the animals on the low-Na diet for at least 48 h. The mean number of open channels per patch (NPo) was maximal after 1 wk on low Na. Channels were also induced within 3 h after injection of furosemide (20 mg\/kg body wt per d). NPo was maximal 48 h after the first injection. In both cases, increases in NPo were primarily due to increases in the number of channels per patch (N) at a constant open probability (Po). With salt depletion or furosemide injection NPo is a saturable function of aldosterone concentration with half-maximal activity at approximately 8 nM. When animals were salt repleted after 1-2 wk of salt depletion, both plasma aldosterone and NPo fell markedly within 6 h. NPo continued to decrease over the next 14 h, while plasma aldosterone rebounded partially. Channel activity may be dissociated from aldosterone concentrations under conditions of salt repletion.<\/jats:p>","DOI":"10.1085\/jgp.102.1.25","type":"journal-article","created":{"date-parts":[[2004,5,13]],"date-time":"2004-05-13T23:51:34Z","timestamp":1084492294000},"page":"25-42","update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":162,"title":["Regulation of Na channels of the rat cortical collecting tubule by aldosterone."],"prefix":"10.1085","volume":"102","author":[{"given":"J","family":"P\u00e1cha","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, Cornell University Medical College, New York 10021."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Frindt","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Cornell University Medical College, New York 10021."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Antonian","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Cornell University Medical College, New York 10021."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R B","family":"Silver","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Cornell University Medical College, New York 10021."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L G","family":"Palmer","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Cornell University Medical College, New York 10021."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1993,7,1]]},"container-title":["The Journal of general physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/102\/1\/25\/1767506\/25.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/102\/1\/25\/1767506\/25.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T10:24:09Z","timestamp":1690367049000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jgp\/article\/102\/1\/25\/26294\/Regulation-of-Na-channels-of-the-rat-cortical"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,7,1]]}},"URL":"https:\/\/doi.org\/10.1085\/jgp.102.1.25","relation":{},"ISSN":["0022-1295","1540-7748"],"issn-type":[{"value":"0022-1295","type":"print"},{"value":"1540-7748","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,7,1]]}}}