{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:27:16Z","timestamp":1779344836574,"version":"3.51.4"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,12,1]]},"abstract":"<jats:p>To understand the role of Na+, K+, and Cl- transporters in fluid and electrolyte secretion by pancreatic acinar cells, we studied the relationship between them in resting and stimulated cells. Measurements of [Cl-]i in resting cells showed that in HCO3(-)-buffered medium [Cl-]i and Cl- fluxes are dominated by the Cl-\/HCO3- exchanger. In the absence of HCO3-, [Cl-]i is regulated by NaCl and NaK2Cl cotransport systems. Measurements of [Na+]i showed that the Na(+)-coupled Cl- transporters contributed to the regulation of [Na+]i, but the major Na+ influx pathway in resting pancreatic acinar cells is the Na+\/H+ exchanger. 86Rb influx measurements revealed that &amp;gt; 95% of K+ influx is mediated by the Na+ pump and the NaK2Cl cotransporter. In resting cells, the two transporters appear to be coupled through [K+]i in that inhibition of either transporter had small effect on 86Rb uptake, but inhibition of both transporters largely prevented 86Rb uptake. Another form of coupling occurs between the Na+ influx transporters and the Na+ pump. Thus, inhibition of NaK2Cl cotransport increased Na+ influx by the Na+\/H+ exchanger to fuel the Na+ pump. Similarly, inhibition of Na+\/H+ exchange increased the activity of the NaK2Cl cotransporter. The combined measurements of [Na+]i and 86Rb influx indicate that the Na+\/H+ exchanger contributes twice more than the NaK2Cl cotransporter and three times more than the NaCl cotransporter and a tetraethylammonium-sensitive channel to Na+ influx in resting cells. These findings were used to develop a model for the relationship between the transporters in resting pancreatic acinar cells.<\/jats:p>","DOI":"10.1085\/jgp.106.6.1225","type":"journal-article","created":{"date-parts":[[2004,5,13]],"date-time":"2004-05-13T23:51:34Z","timestamp":1084492294000},"page":"1225-1242","update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":19,"title":["Na+, K+, and Cl- transport in resting pancreatic acinar cells."],"prefix":"10.1085","volume":"106","author":[{"given":"H","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Muallem","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1995,12,1]]},"container-title":["The Journal of general physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/106\/6\/1225\/1768875\/1225.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/106\/6\/1225\/1768875\/1225.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T11:08:44Z","timestamp":1690369724000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jgp\/article\/106\/6\/1225\/26502\/Na-K-and-Cl-transport-in-resting-pancreatic-acinar"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1995,12,1]]}},"URL":"https:\/\/doi.org\/10.1085\/jgp.106.6.1225","relation":{},"ISSN":["0022-1295","1540-7748"],"issn-type":[{"value":"0022-1295","type":"print"},{"value":"1540-7748","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,12,1]]}}}