{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T20:59:34Z","timestamp":1709585974383},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,5,1]]},"abstract":"<jats:p>By use of microelectrodes, changes in the receptor current and the Ca2+ concentration were measured in the rod layer of the rat retina after stimulation by flashes or steady light. Thereby light induced Ca2+ sources, and sinks along a rod were determined in dependence of time. Thus, the Ca2+ fluxes across the plasma membrane of a mammalian rod could be studied in detail. By light stimulation, Ca2+ sources are evoked along the outer segment only. Immediately after a saturating flash, a maximum of Ca2+ efflux is observed which decays exponentially with tau = 0.3 s at 37 degrees C (4.2 s at 23 degrees C). During regeneration of the dark current, the outer segment acts as a Ca2+ sink, indicating a restoration of the Ca(2+)-depleted outer segment. These findings agree with earlier reports on amphibian rods. Further experiments showed that the peak Ca2+ efflux and tau are temperature dependent. The peak amplitude also depends on the external Ca2+ concentration. In contrast to the reports on amphibian rods, only a part of the Ca2+ ions extruded from the outer segment is directly restored. Surprisingly, during steady light the Ca2+ efflux approaches a permanent residual value. Therefore, in course of a photoresponse, Ca2+ must be liberated irreversibly from internal Ca2+ stores. There is certain evidence that the inner segment acts as a Ca2+ store. Our results show that the Ca2+ fraction of the ions carrying the dark current is proportional to the extracellular Ca2+ concentration. This indicates that the Ca2+ permeability of the plasma membrane of the rod outer segment is independent of the Ca2+ concentration.<\/jats:p>","DOI":"10.1085\/jgp.107.5.577","type":"journal-article","created":{"date-parts":[[2004,5,13]],"date-time":"2004-05-13T23:51:34Z","timestamp":1084492294000},"page":"577-595","update-policy":"http:\/\/dx.doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":4,"title":["Ca2+ fluxes and channel regulation in rods of the albino rat."],"prefix":"10.1085","volume":"107","author":[{"given":"A","family":"Knopp","sequence":"first","affiliation":[{"name":"Max Volmer Institut f\u00fcr Biophysikalische and Physikalische Chemie, Technische Universit\u00e4t Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"R\u00fcppel","sequence":"additional","affiliation":[{"name":"Max Volmer Institut f\u00fcr Biophysikalische and Physikalische Chemie, Technische Universit\u00e4t Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1996,5,1]]},"container-title":["The Journal of general physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/107\/5\/577\/1769560\/577.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/107\/5\/577\/1769560\/577.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T11:16:39Z","timestamp":1690370199000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jgp\/article\/107\/5\/577\/57873\/Ca2-fluxes-and-channel-regulation-in-rods-of-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1996,5,1]]}},"URL":"https:\/\/doi.org\/10.1085\/jgp.107.5.577","relation":{},"ISSN":["0022-1295","1540-7748"],"issn-type":[{"value":"0022-1295","type":"print"},{"value":"1540-7748","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,5,1]]}}}